Rabbit Cofilin 1 (Ser-3) Polyclonal Antibody | anti-CFL1 antibody

Cofilin 1 (Ser-3), phospho-specific

Cat. Num. AAA472016

• Gene Names: CFL1; CFL
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified
• Synonyms: Cofilin 1 (Ser-3); Polyclonal Antibody; phospho-specific; p18; 18 kDa phosphoprotein; anti-CFL1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was cross-adsorbed to unphosphorylated Coflin 1 peptide then affinity purified using phospho-Cofilin 1 (Ser-3) peptide (without carrier). The antibody detects a 19 kDa* protein corresponding to the molecular mass of phosphorylated Cofilin 1 on SDS-PAGE immunoblots of Jurkat cells. This band can be removed by lambda phosphatase treatment.
• Purity/Purification: Affinity Purified
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 166

• Applicable Applications for anti-CFL1 antibody: ELISA, Western Blot
• Application Notes: ELISA: 1:2000; Immunocytochemistry: 1:50; Western Blot: 1:1000
• Immunogen: A synthetic phospho-peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 3 in human Cofilin 1. This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has two amino acid differences from human Cofilin 2.
• Preparation and Storage: Store at -20 degree C. Do not aliquot. Stable for 1 year.

Testing Data

Testing Data 2

Related Product Information for anti-CFL1 antibody

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (Cofilin 2), non-muscle cofilin (Cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity.

References

• Bamburg, J.R. (1999). Annu Rev Cell Dev Biol. 15:185
• Maciver, S.K. & Hussey, P.J. (2002). Genome Biol. 3(5): 3007

NCBI and Uniprot Product Information

• NCBI GI #: 116848
• NCBI GeneID: 1072
• NCBI Accession #: P23528.3
• NCBI GenBank Nucleotide #: P23528
• UniProt Accession #: P23528; Q53Y87; Q9UCA2; B3KUQ1
• Molecular Weight: 18,502 Da
• NCBI Official Full Name: Cofilin-1
• NCBI Official Synonym Full Names: cofilin 1 (non-muscle)
• NCBI Official Symbol: CFL1
• NCBI Official Synonym Symbols: CFL
• NCBI Protein Information: cofilin-1; p18; OTTHUMP00000233679; OTTHUMP00000233680; OTTHUMP00000233681; OTTHUMP00000233682; OTTHUMP00000233740; OTTHUMP00000233741; OTTHUMP00000233742; 18 kDa phosphoprotein; cofilin, non-muscle isoform
• UniProt Protein Name: Cofilin-1
• Protein Family: Cofilin
• UniProt Gene Name: CFL1
• UniProt Synonym Gene Names: CFL
• UniProt Entry Name: COF1_HUMAN

NCBI Description

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Uniprot Description

Function: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Subcellular location: Nucleus matrix. Cytoplasm › cytoskeleton. Note: Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

Tissue specificity: Widely distributed in various tissues.

Induction: Up-regulated in response to enterovirus 71 (EV71) infection (at protein level). Ref.15

Post-translational modification: Phosphorylated on Ser-3 in resting cells

By similarity. Ref.12 Ref.13 Ref.14 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24

Sequence similarities: Belongs to the actin-binding proteins ADF family.Contains 1 ADF-H domain.