Rabbit anti-Human CLK2 Polyclonal Antibody | anti-CLK2 antibody

CLK2 (Dual Specificity Protein Kinase CLK2, CDC-like Kinase 2) (FITC)

Cat. Num. AAA6374140

• Gene Names: CLK2; hCLK2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLK2; Polyclonal Antibody; CLK2 (Dual Specificity Protein Kinase CLK2; CDC-like Kinase 2) (FITC); anti-CLK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLK2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CLK2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human CLK2, aa1-498 (NP_003984.2).
• Immunogen Sequence: MPHPRRYHSSERGSRGSYREHYRSRKHKRRRSRSWSSSSDRTRRRRREDSYHVRSRSSYDDRSSDRRVYDRRYCGSYRRNDYSRDRGDAYYDTDYRHSYEYQRENSSYRSQRSSRRKHRRRRRRSRTFSRSSSHSSRRAKSVEDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRKQKYFYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEEHHQLFDLIESMLEYEPAKRLTLGEALQHPFFARLRAEPPNKLWDSSRDISR
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CLK2 rabbit polyclonal antibody. Western Blot analysis of CLK2 expression in A-431.)

Western Blot (WB)

(Western Blot analysis of CLK2 expression in transfected 293T cell line by CLK2 polyclonal antibody. Lane 1: CLK2 transfected lysate (60kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-CLK2 antibody

This gene encodes a member of the CLK family of dual specificity protein kinases. CLK family members have shown to interact with, and phosphorylate, serine-and arginine-rich (SR) proteins of the spliceosomal complex, which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. This protein kinase is involved in the regulation of several cellular processes and may serve as a link between cell cycle progression, apoptosis, and telomere length regulation.

Product Categories/Family for anti-CLK2 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 47717134
• NCBI GeneID: 1196
• NCBI Accession #: NP_003984.2
• NCBI GenBank Nucleotide #: NM_003993.2
• UniProt Accession #: P49760; Q96CQ0; B1AVS9; B5MBX6
• Molecular Weight: 60,090 Da
• NCBI Official Full Name: dual specificity protein kinase CLK2
• NCBI Official Synonym Full Names: CDC-like kinase 2
• NCBI Official Symbol: CLK2
• NCBI Official Synonym Symbols: hCLK2
• NCBI Protein Information: dual specificity protein kinase CLK2; CLK kinase
• UniProt Protein Name: Dual specificity protein kinase CLK2
• Protein Family: Dual specificity protein kinase
• UniProt Gene Name: CLK2
• UniProt Entry Name: CLK2_HUMAN

NCBI Description

This gene encodes a member of the CLK family of dual specificity protein kinases. CLK family members have been shown to interact with, and phosphorylate, serine- and arginine-rich (SR) proteins of the spliceosomal complex, which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. Note that this gene is distinct from TELO2 gene (GeneID:9894), which shares CLK2 and hCLK2 symbol aliases in common with this gene, but encodes a protein that is involved in telomere length regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

CLK2: Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, CMGC; EC 2.7.12.1; CMGC group; CLK family

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: response to retinoic acid; peptidyl-tyrosine phosphorylation; regulation of RNA splicing; protein amino acid autophosphorylation; negative regulation of gluconeogenesis; response to ionizing radiation; protein amino acid phosphorylation