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Immunofluorescence (IF) (Fig.1. Immunofluorescence staining (1:200) of mCherry fusion protein in 293 cells with red.)

Mouse mCherry Tag Monoclonal Antibody

Anti-mCherry Tag Mouse Monoclonal Antibody (9D3)

Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Synonyms
mCherry Tag; Monoclonal Antibody; Anti-mCherry Tag Mouse Monoclonal Antibody (9D3); mCherry; mCherry tag; anti-mCherry Tag antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Form/Format
Liquid in PBS, pH7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Applicable Applications for anti-mCherry Tag antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:5000
IF: 1:200
Tag
mCherry
Immunogen
Recombinant Protein
Preparation and Storage
Stable for one year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping||Gel pack with blue ice.

Immunofluorescence (IF)

(Fig.1. Immunofluorescence staining (1:200) of mCherry fusion protein in 293 cells with red.)

Immunofluorescence (IF) (Fig.1. Immunofluorescence staining (1:200) of mCherry fusion protein in 293 cells with red.)

Western Blot (WB)

(Fig.2. Western blot analysis of 1ug mCherry fusion protein with Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) in 1:2000 (lane A) and 1:5000 (lane B) dilutions.)

Western Blot (WB) (Fig.2. Western blot analysis of 1ug mCherry fusion protein with Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) in 1:2000 (lane A) and 1:5000 (lane B) dilutions.)
Related Product Information for anti-mCherry Tag antibody
Description: Featured Anti-mCherry Tag Mouse Monoclonal Antibody (9D3), specially designed for your taged protein quantification and localization.
Background: mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry, derived from a protein isolated from Discosoma sp., is a 28.8kD monomeric fluorescent construct with peak absorption/emission at 587nm and 610nm. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores.
Product Categories/Family for anti-mCherry Tag antibody

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Product Notes

The mCherry Tag (Catalog #AAA9719149) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's mCherry Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:5000 IF: 1:200. Researchers should empirically determine the suitability of the mCherry Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mCherry Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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