Western Blot (WB)
(Western blot analysis of Ryanodine Receptor 2 (pS2808) expression in U251 (A) whole cell lysates.)
Rabbit Ryanodine Receptor 2 Polyclonal Antibody | anti-RYR2 antibody
Anti-Ryanodine Receptor 2 (pS2808) Phospho Antibody
Western Blot (WB)
(Western blot analysis of Ryanodine Receptor 2 (pS2808) expression in U251 (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Ryanodine Receptor 2 (pS2808) expression in U251 (A) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Ryanodine Receptor 2 (pS2808) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Ryanodine Receptor 2 (pS2808) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Ryanodine Receptor 2 (pS2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of Ryanodine Receptor 2 (pS2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
ELISA
(Direct ELISA antibody dose-response curve using Anti-Ryanodine Receptor 2 (pS2808) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.)
ELISA
(Direct ELISA antibody dose-response curve using Anti-Ryanodine Receptor 2 (pS2808) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. [provided by RefSeq, Jul 2008]
Uniprot Description
RYR2: ryanodine receptor calcium release channel. Regulates communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Phosphorylation by PKA dissociates FKBP12.6 from RYR2. Two splice isoforms have been described.
Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Transporter, ion channel; Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q43
Cellular Component: sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; protein complex; membrane; sarcoplasmic reticulum; plasma membrane; Z disc
Molecular Function: calmodulin binding; calcium-induced calcium release activity; identical protein binding; protein binding; enzyme binding; protein self-association; calcium-release channel activity; calcium channel activity; calcium ion binding; intracellular ligand-gated calcium channel activity; ryanodine-sensitive calcium-release channel activity
Biological Process: positive regulation of sequestering of calcium ion; cytosolic calcium ion homeostasis; regulation of heart rate; calcium-mediated signaling; positive regulation of heart rate; response to redox state; Wnt receptor signaling pathway through beta-catenin; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; response to caffeine; cellular calcium ion homeostasis; BMP signaling pathway; calcium ion transport; release of sequestered calcium ion into cytosol; response to hypoxia; detection of calcium ion; transmembrane transport; cardiac muscle contraction
Disease: Ventricular Tachycardia, Catecholaminergic Polymorphic, 1, With Or Without Atrial Dysfunction And/or Dilated Cardiomyopathy; Arrhythmogenic Right Ventricular Dysplasia, Familial, 2
Research Articles on RYR2
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Product Notes
The RYR2 ryr2 (Catalog #AAA8236073) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ryanodine Receptor 2 (pS2808) Phospho Antibody reacts with Human, Mouse, Rat, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Ryanodine Receptor 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB),Immunohistochemistry (IHC), Immunofluoresence/Immunocytochemistry (IF/ICC). WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/ICC (1/100 - 1/500). Researchers should empirically determine the suitability of the RYR2 ryr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ryanodine Receptor 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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