Western Blot (WB)
(CLK2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CLK2 antibody detected the CLK2 protein (arrow).)
Rabbit CLK2 Polyclonal Antibody | anti-CLK2 antibody
CLK2 Antibody (Center)
Western Blot (WB)
(CLK2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CLK2 antibody detected the CLK2 protein (arrow).)
Western Blot (WB)
(CLK2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CLK2 antibody detected the CLK2 protein (arrow).)
specificity protein kinases. CLK family members have been shown to
interact with, and phosphorylate, serine- and arginine-rich (SR)
proteins of the spliceosomal complex, which is a part of the
regulatory mechanism that enables the SR proteins to control RNA
splicing. Note that this gene is distinct from TELO2 gene
(GeneID:9894), which shares CLK2 and hCLK2 symbol aliases in common
with this gene, but encodes a protein that is involved in telomere
length regulation.
Eisenreich, A., et al. Circ. Res. 104(5):589-599(2009)
Rendtlew Danielsen, J.M., et al. J. Biol. Chem. 284(7):4140-4147(2009)
Castle, J.C., et al. Nat. Genet. 40(12):1416-1425(2008)
Jin, J., et al. Curr. Biol. 14(16):1436-1450(2004)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Uniprot Description
CLK2: Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, dual-specificity (non-receptor); EC 2.7.12.1; Kinase, protein; Protein kinase, CMGC; CMGC group; CLK family
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: nucleoplasm; nuclear speck; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding
Biological Process: response to retinoic acid; peptidyl-tyrosine phosphorylation; regulation of RNA splicing; negative regulation of gluconeogenesis; protein amino acid autophosphorylation; response to ionizing radiation; protein amino acid phosphorylation
Research Articles on CLK2
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Product Notes
The CLK2 clk2 (Catalog #AAA9210168) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 291-319. The CLK2 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's CLK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the CLK2 clk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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