Rabbit anti-Human CLIP3 Polyclonal Antibody | anti-CLIP3 antibody

CLIP3, CT (CLIP3, CLIPR59, CAP-Gly domain-containing linker protein 3, Cytoplasmic linker protein 170-related 59kD protein) (APC)

Cat. Num. AAA6286768

• Gene Names: CLIP3; RSNL1; CLIPR59; CLIPR-59
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLIP3; Polyclonal Antibody; CT (CLIP3; CLIPR59; CAP-Gly domain-containing linker protein 3; Cytoplasmic linker protein 170-related 59kD protein) (APC); Cytoplasmic linker protein 170-related 59kD protein; anti-CLIP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 547

• Applicable Applications for anti-CLIP3 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CLIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368-396 amino acids from the C-terminal region of human CLIP3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CLIP3 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CLIP3 antibody detected the CLIP3 protein (arrow).)

Flow Cytometry (FC/FACS)

(CLIP3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CLIP3 antibody

Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics.

Product Categories/Family for anti-CLIP3 antibody

Antibodies; Proteins

References

Sorice, M., et al. PLoS ONE 5 (1), E8567 (2010): Ding, J., et al. Mol. Cell. Biol. 29(6):1459-1471(2009). Lallemand-Breitenbach, V., et al. J. Biol. Chem. 279(39):41168-41178(2004). Perez, F., et al. J. Cell Biol. 156(4):631-642(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 18496983
• NCBI GeneID: 25999
• NCBI Accession #: NP_056341.1
• NCBI GenBank Nucleotide #: NP_056341.1
• UniProt Accession #: Q96DZ5
• NCBI Official Full Name: CAP-Gly domain-containing linker protein 3
• NCBI Official Synonym Full Names: CAP-Gly domain containing linker protein 3
• NCBI Official Symbol: CLIP3
• NCBI Official Synonym Symbols: RSNL1; CLIPR59; CLIPR-59
• NCBI Protein Information: CAP-Gly domain-containing linker protein 3
• UniProt Protein Name: CAP-Gly domain-containing linker protein 3
• Protein Family: CAP-Gly domain-containing linker protein
• UniProt Gene Name: CLIP3
• UniProt Synonym Gene Names: CLIPR59; CLIP-170-related 59 kDa protein; CLIPR-59
• UniProt Entry Name: CLIP3_HUMAN

NCBI Description

This gene encodes a member of the cytoplasmic linker protein 170 family. Members of this protein family contain a cytoskeleton-associated protein glycine-rich domain and mediate the interaction of microtubules with cellular organelles. The encoded protein plays a role in T cell apoptosis by facilitating the association of tubulin and the lipid raft ganglioside GD3. The encoded protein also functions as a scaffold protein mediating membrane localization of phosphorylated protein kinase B. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CLIPR-59: Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: Golgi stack; early endosome membrane; recycling endosome membrane; plasma membrane; trans-Golgi network membrane; trans-Golgi network; lipid raft

Molecular Function: microtubule binding

Biological Process: peptidyl-S-palmitoyl-L-cysteine biosynthetic process from peptidyl-cysteine; fat cell differentiation; positive regulation of apoptosis; negative regulation of microtubule polymerization; positive regulation of endocytosis; positive regulation of protein amino acid phosphorylation