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Western Blot (WB)
(Western blot analysis of CdGAP (Phospho-Thr789) Antibody expression in Insulin treated Hela cells lysates..-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit CdGAP Polyclonal Antibody | anti-CdGAP antibody
Phospho-CdGAP (Thr789) Antibody
Gene Names
ARHGAP31; AOS1; CDGAP
Reactivity
Human, Mouse, RatPredicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
CdGAP; Polyclonal Antibody; Phospho-CdGAP (Thr789) Antibody; ARHGAP31; Cdc42 GTPase-activating protein; RHG31_HUMAN; Rho GTPase-activating protein 31; anti-CdGAP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-CdGAP (Thr789) Antibody detects endogenous levels of CdGAP only when phosphorylated at Thr789.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CdGAP antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human CdGAP around the phosphorylation site of Thr789.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylation on Thr-789 reduces GAP activity.
Subunit Structure
Interacts with ITSN1, which inhibits GAP activity. Interacts with PARVA (By similarity). Interacts with GTP-loaded RHOU.
Subcellular Location
Cell projection>Lamellipodium. Cell junction>Focal adhesion.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of CdGAP (Phospho-Thr789) Antibody expression in Insulin treated Hela cells lysates..-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of CdGAP (Phospho-Thr789) Antibody expression in Insulin treated Hela cells lysates..-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Immunohistochemistry (IHC)
(Staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-CdGAP antibody
Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
156,985 Da
NCBI Official Full Name
rho GTPase-activating protein 31
NCBI Official Synonym Full Names
Rho GTPase activating protein 31
NCBI Official Symbol
ARHGAP31
NCBI Official Synonym Symbols
AOS1; CDGAP
NCBI Protein Information
rho GTPase-activating protein 31; Cdc42 GTPase-activating protein
UniProt Protein Name
Rho GTPase-activating protein 31
UniProt Gene Name
ARHGAP31
UniProt Synonym Gene Names
CDGAP; KIAA1204
Uniprot Description
Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.
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Product Notes
The CdGAP arhgap31 (Catalog #AAA9614253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CdGAP (Thr789) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's CdGAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:1000-3000 IHC: 1:50-1:200 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the CdGAP arhgap31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CdGAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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