Chicken CLC3 Polyclonal Antibody | anti-CLCN3 antibody

CLC3 (Clcn3, Chloride Channel)

Cat. Num. AAA615244

• Gene Names: CLCN3; CLC3; ClC-3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CLC3; Polyclonal Antibody; CLC3 (Clcn3; Chloride Channel); Anti -CLC3 (Clcn3; anti-CLCN3 antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human Chloride Intercellular channel 3. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.

• Applicable Applications for anti-CLCN3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:500
• Immunogen: Synthetic peptide corresponding to aa29-236
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Test: Human Placenta protein extract as test antigen, loaded 70ug per lane. MBS615244 dilution: 1:500, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)

Related Product Information for anti-CLCN3 antibody

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 3 is a member of the p64 family and is predominantly localized in the nucleus and stimulates chloride ion channel activity. In addition, this protein may participate in cellular growth control, based on its association with ERK7, a member of the MAP kinase family.

Product Categories/Family for anti-CLCN3 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 223460504
• NCBI GeneID: 1182
• UniProt Accession #: P51790; O14918; Q86Z21; D3DP34; Q7Z4M4
• Molecular Weight: 90,966 Da
• NCBI Official Full Name: CLCN3 protein
• NCBI Official Synonym Full Names: chloride channel 3
• NCBI Official Symbol: CLCN3
• NCBI Official Synonym Symbols: CLC3; ClC-3
• NCBI Protein Information: H(+)/Cl(-) exchange transporter 3; H(+)/Cl(-) exchange transporter 3; OTTHUMP00000219460; OTTHUMP00000219503; OTTHUMP00000219505; OTTHUMP00000219506; chloride channel protein 3; chloride transporter ClC-3
• UniProt Protein Name: H(+)/Cl(-) exchange transporter 3
• Protein Family: H(+)/Cl(-) exchange transporter
• UniProt Gene Name: CLCN3
• UniProt Entry Name: CLCN3_HUMAN

NCBI Description

This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CLCN3: Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory. Belongs to the chloride channel (TC 2.A.49) family. ClC-3/CLCN3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Vesicle; Transporter; Channel, chloride; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q33

Cellular Component: Golgi apparatus; specific granule; cell surface; late endosome membrane; early endosome; integral to membrane; vesicle membrane; phagocytic vesicle; secretory granule; Golgi membrane; synaptic vesicle; membrane; early endosome membrane; apical plasma membrane; late endosome; plasma membrane; cytoplasmic vesicle; external side of plasma membrane

Molecular Function: protein binding; chloride channel activity; protein homodimerization activity; protein heterodimerization activity; voltage-gated chloride channel activity; ATP binding; PDZ domain binding; antiporter activity

Biological Process: transport; regulation of pH; negative regulation of cell volume; transmembrane transport; endosomal lumen acidification