Rabbit anti-Human CLCN3 Polyclonal Antibody | anti-CLCN3 antibody

CLCN3, ID (CLCN3, H(+)/Cl(-) exchange transporter 3, Chloride channel protein 3, Chloride transporter ClC-3)

Cat. Num. AAA6009846

• Gene Names: CLCN3; CLC3; ClC-3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CLCN3; Polyclonal Antibody; ID (CLCN3; H(+)/Cl(-) exchange transporter 3; Chloride channel protein 3; Chloride transporter ClC-3); Anti -CLCN3; anti-CLCN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CLCN3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: CLCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-313 amino acids from the Central region of human CLCN3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CLCN3 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the CLCN3 antibody detected the CLCN3 protein (arrow).)

Related Product Information for anti-CLCN3 antibody

CLCN3 mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.

Product Categories/Family for anti-CLCN3 antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 223460504
• NCBI GeneID: 1182
• UniProt Accession #: P51790; O14918; Q86Z21; B7Z932; D3DP34
• Molecular Weight: 90,966 Da
• NCBI Official Full Name: CLCN3 protein
• NCBI Official Synonym Full Names: chloride channel, voltage-sensitive 3
• NCBI Official Symbol: CLCN3
• NCBI Official Synonym Symbols: CLC3; ClC-3
• NCBI Protein Information: H(+)/Cl(-) exchange transporter 3; H(+)/Cl(-) exchange transporter 3; chloride channel 3; chloride channel protein 3; chloride transporter ClC-3
• UniProt Protein Name: H(+)/Cl(-) exchange transporter 3
• Protein Family: H(+)/Cl(-) exchange transporter
• UniProt Gene Name: CLCN3
• UniProt Synonym Gene Names: ClC-3
• UniProt Entry Name: CLCN3_HUMAN

NCBI Description

This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CLCN3: Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory. Belongs to the chloride channel (TC 2.A.49) family. ClC-3/CLCN3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Channel, chloride; Membrane protein, multi-pass; Transporter; Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q33

Cellular Component: Golgi apparatus; specific granule; cell surface; late endosome membrane; early endosome; integral to membrane; phagocytic vesicle; vesicle membrane; secretory granule; Golgi membrane; synaptic vesicle; membrane; early endosome membrane; apical plasma membrane; late endosome; plasma membrane; cytoplasmic vesicle; external side of plasma membrane

Molecular Function: protein binding; chloride channel activity; protein homodimerization activity; protein heterodimerization activity; voltage-gated chloride channel activity; ATP binding; antiporter activity; PDZ domain binding

Biological Process: transport; regulation of pH; negative regulation of cell volume; transmembrane transport; endosomal lumen acidification