Rabbit anti-Human CKMT1A/CKMT1B Polyclonal Antibody | anti-CKMT1A/CKMT1B antibody

CKMT1A/CKMT1B Polyclonal Antibody

Cat. Num. AAA2561190

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Antigen Affinity Purification
• Synonyms: CKMT1A/CKMT1B; Polyclonal Antibody; CKMT1A/CKMT1B Polyclonal Antibody; Acidic-type mitochondrial creatine kinase; CKMT; CKMT1; CKMT1B; Creatine kinase mitochondrial 1 (ubiquitous); Creatine kinase mitochondrial 1B; Creatine kinase U type mitochondrial; Creatine kinase U-type; KCRU; Mia-CK; Mitochondrial; U-MtCK; Ubiquitous mitochondrial creatine kinase; UMTCK; anti-CKMT1A/CKMT1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
• Concentration: 1.68mg/mL (varies by lot)
• Sequence Length: 417

• Applicable Applications for anti-CKMT1A/CKMT1B antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:40-1:200; ELISA: 1:5000-1:10000
• Immunogen: Synthetic peptide of human CKMT1A/CKMT1B
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of MCF7 cell lysate using CKMT1A/CKMT1B Polyclonal Antibody at dilution of 1:600)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using CKMT1A/CKMT1B Polyclonal Antibody at dilution of 1:55(×200))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human tonsil tissue using CKMT1A/CKMT1B Polyclonal Antibody at dilution of 1:55(×200))

Related Product Information for anti-CKMT1A/CKMT1B antibody

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 66346730
• NCBI Accession #: NP_001015001.1
• NCBI GenBank Nucleotide #: NP_001015001.1
• UniProt Accession #: P12532
• Molecular Weight: 47 kDa
• NCBI Official Full Name: creatine kinase U-type, mitochondrial isoform 1
• UniProt Protein Name: Creatine kinase U-type, mitochondrial
• UniProt Gene Name: CKMT1A
• UniProt Synonym Gene Names: CKMT; Mia-CK; U-MtCK
• UniProt Entry Name: KCRU_HUMAN

Uniprot Description

CKMT1A: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Exists as an octamer composed of four MTCK homodimers. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Kinase, other; EC 2.7.3.2; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphorylation; creatine metabolic process