Rabbit CIP75 Polyclonal Antibody | anti-UBQLN4 antibody

CIP75 Antibody

Cat. Num. AAA151318

• Gene Names: UBQLN4; A1U; A1Up; UBIN; CIP75; C1orf6
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: CIP75 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CIP75; Polyclonal Antibody; CIP75 Antibody; A1U; A1Up; UBIN; C1orf6; Ubiquilin-4; Ataxin-1 interacting ubiquitin-like protein; ubiquilin 4; anti-UBQLN4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CIP75 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 601

• Applicable Applications for anti-UBQLN4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: CIP75 antibody can be used for detection of CIP75 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CIP75 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CIP75.
• Buffer: CIP75 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CIP75 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CIP75 in 3T3 cell lysate with CIP75 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CIP75 in mouse kidney tissue with CIP75 antibody at 5 μg/mL.)

Related Product Information for anti-UBQLN4 antibody

CIP75 Antibody: The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 45476982
• NCBI GeneID: 56893
• UniProt Accession #: Q9NRR5; Q5VYA0; Q5VYA1; Q9BR98; Q9UHX4; A6ND44; B2RAY7
• Molecular Weight: 24,527 Da
• NCBI Official Full Name: Ubiquilin-4
• NCBI Official Synonym Full Names: ubiquilin 4
• NCBI Official Symbol: UBQLN4
• NCBI Official Synonym Symbols: A1U; A1Up; UBIN; CIP75; C1orf6
• NCBI Protein Information: ubiquilin-4; connexin43-interacting protein of 75 kDa; ataxin-1 interacting ubiquitin-like protein; ataxin-1 ubiquitin-like interacting protein; ataxin-1 ubiquitin-like-interacting protein A1U
• UniProt Protein Name: Ubiquilin-4
• Protein Family: Ubiquilin
• UniProt Gene Name: UBQLN4
• UniProt Synonym Gene Names: C1orf6; CIP75; UBIN; A1Up; CIP75
• UniProt Entry Name: UBQL4_HUMAN

Uniprot Description

UBQLN4: Plays a role in the regulation of proteasomal protein degradation. Depending on the case, may promote or inhibit proteasomal protein degradation. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: endoplasmic reticulum membrane; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; polyubiquitin binding

Biological Process: regulation of proteasomal ubiquitin-dependent protein catabolic process