Typical Standard Curve/Testing Data
Bovine C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8 (CPAMD8) ELISA Kit | CPAMD8 elisa kit
Bovine C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8 (CPAMD8) ELISA Kit
Gene Names
CPAMD8; VIP; K-CAP
Reactivity
Bovine
Synonyms
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8 (CPAMD8); Bovine C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8 (CPAMD8) ELISA Kit; CPAMD8 elisa kit
Reactivity
Bovine
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for CPAMD8 elisa kit
Intended Uses: This CPAMD8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine CPAMD8. This ELISA kit for research use only!
Principle of the Assay: CPAMD8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CPAMD8 antibody and an CPAMD8-HRP conjugate. The assay sample and buffer are incubated together with CPAMD8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPAMD8 concentration since CPAMD8 from samples and CPAMD8-HRP conjugate compete for the anti-CPAMD8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPAMD8 from the sample, fewer sites are left to bind CPAMD8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPAMD8 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CPAMD8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CPAMD8 antibody and an CPAMD8-HRP conjugate. The assay sample and buffer are incubated together with CPAMD8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPAMD8 concentration since CPAMD8 from samples and CPAMD8-HRP conjugate compete for the anti-CPAMD8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPAMD8 from the sample, fewer sites are left to bind CPAMD8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPAMD8 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CPAMD8 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
199,171 Da
NCBI Official Full Name
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8
NCBI Official Synonym Full Names
C3 and PZP-like, alpha-2-macroglobulin domain containing 8
NCBI Official Symbol
CPAMD8
NCBI Official Synonym Symbols
VIP; K-CAP
NCBI Protein Information
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8; alpha-2 macroglobulin family protein VIP
UniProt Protein Name
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8
UniProt Gene Name
CPAMD8
UniProt Synonym Gene Names
KIAA1283
UniProt Entry Name
CPMD8_HUMAN
NCBI Description
CPAMD8 belongs to the complement component-3 (C3; MIM 120700)/alpha-2-macroglobulin (A2M; MIM 103950) family of proteins, which are involved in innate immunity and damage control. Complement components recognize and eliminate pathogens by direct binding or by mediating opsonization/phagocytosis and intracellular killing, and A2M is a broad-spectrum protease inhibitor (Li et al., 2004 [PubMed 15177561]).[supplied by OMIM, Mar 2008]
Uniprot Description
CPAMD8: Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. Up-regulated by IL1B/interleukin-1 beta and IL6/interleukin-6. Interacts with heparin. 2 isoforms of the human protein are produced by alternative splicing
Chromosomal Location of Human Ortholog: 19p13.11
Cellular Component: extracellular space; plasma membrane
Molecular Function: serine-type endopeptidase inhibitor activity
Research Articles on CPAMD8
Similar Products
Product Notes
The Bovine CPAMD8 cpamd8 (Catalog #AAA7212160) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7212160 ELISA Kit recognizes Bovine CPAMD8. It is sometimes possible for the material contained within the vial of "C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8 (CPAMD8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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