Rabbit anti-Human, Mouse DMP1 Polyclonal Antibody | anti-DMP1 antibody

DMP1 Polyclonal Antibody

Cat. Num. AAA3011421

• Gene Names: DMP1; ARHP; ARHR; DMP-1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DMP1; Polyclonal Antibody; DMP1 Polyclonal Antibody; ARHP; ARHR; AV020965; Dentin matrix acidic phosphoprotein 1; Dentin matrix protein 1; DENTMAT; DMP 1; Dmp; MGC130441; PP; Serine rich acidic phosphoprotein; anti-DMP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DMP1 polyclonal antibody detects endogenous levels of DMP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 497

• Applicable Applications for anti-DMP1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human DMP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DMP1 polyclonal antibody)

Related Product Information for anti-DMP1 antibody

DMP-1 (dentin matrix protein-1), also known as dentin matrix acidic phosphoprotein-1, is a member of the small integrin ligand N-linked glycoprotein family. It is important for the mineralization of bone and dentin. DMP-1 is expressed in bone, tooth and hypertrophic cartilage. It is synthesized by preosteoblasts and contains a large number of acidic domains. DMP-1 localizes to the nucleus of undifferentiated osteoblasts where it functions as a transcriptional regulator for osteoblast-specific gene activation and induces osteoblast differentiation. During osteoblast maturation, DMP-1 undergoes a conformational change and becomes phosphorylated by casein kinase II in response to an influx of calcium ions to the nucleus. DMP-1 is then exported to the extracellular matrix (ECM) where it regulates the nucleation of hydroxyapatite and the formation of calcified tissue. DMP-1 is proteolytically processed into N- and C-terminal fragments in the ECM of bone and dentin. The protein has also been identified in bone as a high molecular weight proteoglycan comprised of the N-terminal DMP-1 fragment and chondroitin sulfate.The loss of DMP-1 can result in hypomineralized bone.

NCBI and Uniprot Product Information

• NCBI GI #: 121256621
• NCBI GeneID: 1758
• NCBI Accession #: NP_001073380.1
• NCBI GenBank Nucleotide #: NP_001073380.1
• UniProt Accession #: Q13316
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: dentin matrix acidic phosphoprotein 1 isoform 2
• NCBI Official Synonym Full Names: dentin matrix acidic phosphoprotein 1
• NCBI Official Symbol: DMP1
• NCBI Official Synonym Symbols: ARHP; ARHR; DMP-1
• NCBI Protein Information: dentin matrix acidic phosphoprotein 1
• UniProt Protein Name: Dentin matrix acidic phosphoprotein 1
• Protein Family: Dentin matrix acidic phosphoprotein
• UniProt Gene Name: DMP1
• UniProt Synonym Gene Names: DMP-1; Dentin matrix protein 1
• UniProt Entry Name: DMP1_HUMAN

NCBI Description

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.1 PublicationManual assertion based on experiment in:Ref.6