Rabbit anti-Human CIDEC Polyclonal Antibody | anti-CIDEC antibody

CIDEC Antibody

Cat. Num. AAA9609230

• Gene Names: CIDEC; CIDE3; FPLD5; FSP27; CIDE-3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CIDEC; Polyclonal Antibody; CIDEC Antibody; Cell death activator; Cell death activator CIDE-3; Cell death inducing DFFA like effector C; Cell death inducing DFFA like effector protein C; Cell death-inducing DFFA-like effector protein C; CIDE 3; CIDE3; CIDEC_HUMAN; Fat specific protein 27; Fat-specific protein FSP27 homolog; FLJ20871; FPLD5; FSP27; anti-CIDEC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CIDEC antibody detects endogenous levels of total CIDEC
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 248

• Applicable Applications for anti-CIDEC antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human CIDEC
• Subcellular Location: Cytoplasm. Cytoplasmic in a punctate manner.
• Tissue Specificity: Expressed mainly in adipose tissue, small intestine, heart, colon and stomach and, at lower levels, in brain, kidney and liver.
• Predicted Cross Reactivity: Horse
• Similarity: Horse (83%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of CIDEC using HeLa whole cell lysates)

Related Product Information for anti-CIDEC antibody

Function: Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1. May act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment. As mature adipocytes, that express high CIDEC levels, are quite resistant to apoptotic stimuli, the physiological significance of its role in apoptosis is unclear. May play a role in the modulation of the response to osmotic stress by preventing NFAT5 to translocate into the nucleus and activate its target genes expression.
Subunit Structure: Homodimer (By similarity). Interacts with CEBPB. Interacts with NFAT5; this interaction is direct and retains NFAT5 in the cytoplasm (By similarity). Interacts with CIDEA. Interacts with PLIN1.
Post-translational Modifications: Ubiquitinated and targeted to proteasomal degradation, resulting in a short half-life. Protein stability depends on triaclyglycerol synthesis, fatty acid availability and lipid droplet formation (By similarity).
Similarity: The CIDE-N domain is involved in homodimerization which is crucial for its function in promoting lipid exchange and transfer.

NCBI and Uniprot Product Information

• NCBI GI #: 313851088
• NCBI GeneID: 63924
• NCBI Accession #: NP_001186480.1
• NCBI GenBank Nucleotide #: NM_001199551.1
• UniProt Accession #: Q96AQ7; Q67DW9; Q9GZY9; C9JMN7
• Molecular Weight: Observed: 26 kDa; Predicted: 27 kDa
• NCBI Official Full Name: cell death activator CIDE-3 isoform 2
• NCBI Official Synonym Full Names: cell death inducing DFFA like effector c
• NCBI Official Symbol: CIDEC
• NCBI Official Synonym Symbols: CIDE3; FPLD5; FSP27; CIDE-3
• NCBI Protein Information: cell death activator CIDE-3
• UniProt Protein Name: Cell death activator CIDE-3
• Protein Family: Cell death activator
• UniProt Gene Name: CIDEC
• UniProt Synonym Gene Names: FSP27

NCBI Description

This gene encodes a member of the cell death-inducing DNA fragmentation factor-like effector family. Members of this family play important roles in apoptosis. The encoded protein promotes lipid droplet formation in adipocytes and may mediate adipocyte apoptosis. This gene is regulated by insulin and its expression is positively correlated with insulin sensitivity. Mutations in this gene may contribute to insulin resistant diabetes. A pseudogene of this gene is located on the short arm of chromosome 3. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1. May act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment. As mature adipocytes, that express high CIDEC levels, are quite resistant to apoptotic stimuli, the physiological significance of its role in apoptosis is unclear. May play a role in the modulation of the response to osmotic stress by preventing NFAT5 to translocate into the nucleus and activate its target genes expression.