Human Cell death activator CIDE A (CIDEA) ELISA Kit | CIDEA elisa kit

Human Cell death activator CIDE A (CIDEA) ELISA Kit

Cat. Num. AAA7241033

• Gene Names: CIDEA; CIDE-A
• Reactivity: Human
• Synonyms: Cell death activator CIDE A (CIDEA); Human Cell death activator CIDE A (CIDEA) ELISA Kit; CIDEA elisa kit

• Reactivity: Human

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for CIDEA elisa kit

Intended Uses: This CIDEA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CIDEA. This ELISA kit for research use only!

Principle of the Assay: CIDEA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CIDEA antibody and an CIDEA-HRP conjugate. The assay sample and buffer are incubated together with CIDEA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CIDEA concentration since CIDEA from samples and CIDEA-HRP conjugate compete for the anti-CIDEA antibody binding site. Since the number of sites is limited, as more sites are occupied by CIDEA from the sample, fewer sites are left to bind CIDEA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CIDEA concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557465
• NCBI GeneID: 1149
• NCBI Accession #: NP_001270.1
• NCBI GenBank Nucleotide #: NM_001279.3
• Molecular Weight: 24,687 Da
• NCBI Official Full Name: cell death activator CIDE-A isoform 1
• NCBI Official Synonym Full Names: cell death-inducing DFFA-like effector a
• NCBI Official Symbol: CIDEA
• NCBI Official Synonym Symbols: CIDE-A
• NCBI Protein Information: cell death activator CIDE-A
• UniProt Protein Name: Cell death activator CIDE-A
• Protein Family: Cell death activator
• UniProt Gene Name: CIDEA
• UniProt Entry Name: CIDEA_HUMAN

NCBI Description

This gene encodes the homolog of the mouse protein Cidea that has been shown to activate apoptosis. This activation of apoptosis is inhibited by the DNA fragmentation factor DFF45 but not by caspase inhibitors. Mice that lack functional Cidea have higher metabolic rates, higher lipolysis in brown adipose tissue and higher core body temperatures when subjected to cold. These mice are also resistant to diet-induced obesity and diabetes. This suggests that in mice this gene product plays a role in thermogenesis and lipolysis. Alternatively spliced transcripts have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

CIDEA: Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin- resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 18p11.21|18

Cellular Component: mitochondrial envelope; mitochondrion; cytoplasm; lipid particle; nucleus

Molecular Function: protein homodimerization activity

Biological Process: cell death; regulation of apoptosis; sequestering of lipid; transcription, DNA-dependent; negative regulation of lipid catabolic process; regulation of transcription, DNA-dependent; apoptosis; negative regulation of cytokine secretion; negative regulation of tumor necrosis factor production; lipid metabolic process; thermoregulation; negative regulation of transforming growth factor beta receptor signaling pathway