Rabbit anti-Human CHMP1A Polyclonal Antibody | anti-CHMP1A antibody

CHMP1A, NT (CHMP1A, CHMP1, KIAA0047, PCOLN3, PRSM1, Charged multivesicular body protein 1a, Chromatin-modifying protein 1a, Vacuolar protein sorting-associated protein 46-1) (FITC)

Cat. Num. AAA6285659

• Gene Names: CHMP1A; PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHMP1A; Polyclonal Antibody; NT (CHMP1A; CHMP1; KIAA0047; PCOLN3; PRSM1; Charged multivesicular body protein 1a; Chromatin-modifying protein 1a; Vacuolar protein sorting-associated protein 46-1) (FITC); Vacuolar protein sorting-associated protein 46-1; anti-CHMP1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CHMP1A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CHMP1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human CHMP1A.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CHMP1A Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the CHMP1A antibody detected the CHMP1A protein (arrow).)

Related Product Information for anti-CHMP1A antibody

This gene encodes a member of the CHMP/Chmp family of proteins which are involved in multivesicular body sorting of proteins to the interiors of lysosomes. The initial prediction of the protein sequence encoded by this gene suggested that the encoded protein was a metallopeptidase. The nomenclature has been updated recently to reflect the correct biological function of this encoded protein.

Product Categories/Family for anti-CHMP1A antibody

Antibodies; Chromatin Proteins

References

Tandon, R., et al. J. Virol. 83(20):10797-10807(2009). Li, J., et al. Cell Cycle 7(18):2886-2893(2008). Row, P.E., et al. J. Biol. Chem. 282(42):30929-30937(2007). Stuchell-Brereton, M.D., et al. Nature 449(7163):740-744(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 103485496
• NCBI GeneID: 5119
• NCBI Accession #: NP_002759.2
• NCBI GenBank Nucleotide #: NM_002768.4
• UniProt Accession #: Q9HD42; Q14468; Q15779; Q96G31; A2RU09
• Molecular Weight: 7,363 Da
• NCBI Official Full Name: charged multivesicular body protein 1a isoform 2
• NCBI Official Synonym Full Names: charged multivesicular body protein 1A
• NCBI Official Symbol: CHMP1A
• NCBI Official Synonym Symbols: PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
• NCBI Protein Information: charged multivesicular body protein 1a; charged multivesicular body protein 1/chromatin modifying protein 1; chromatin modifying protein 1A; procollagen (type III) N-endopeptidase; protease, metallo, 1, 33kD; vacuolar protein sorting-associated protein 46
• UniProt Protein Name: Charged multivesicular body protein 1a
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP1A
• UniProt Synonym Gene Names: CHMP1; KIAA0047; PCOLN3; PRSM1; CHMP1a; Vps46-1; hVps46-1
• UniProt Entry Name: CHM1A_HUMAN

NCBI Description

This gene encodes a member of the CHMP/Chmp family of proteins which are involved in multivesicular body sorting of proteins to the interiors of lysosomes. The initial prediction of the protein sequence encoded by this gene suggested that the encoded protein was a metallopeptidase. The nomenclature has been updated recently to reflect the correct biological function of this encoded protein. Several transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

CHMP1A: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing. Belongs to the SNF7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Protease

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: nuclear matrix; condensed nuclear chromosome; early endosome; microtubule organizing center; endomembrane system

Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; metallopeptidase activity; zinc ion binding

Biological Process: cell separation during cytokinesis; transcription, DNA-dependent; mitotic chromosome condensation; negative regulation of transcription by glucose; vacuolar transport; cytokinesis; gene silencing; proteolysis; mitotic metaphase plate congression; vesicle-mediated transport; protein transport; negative regulation of transcription, DNA-dependent; nuclear organization and biogenesis

Disease: Pontocerebellar Hypoplasia, Type 8