Human Monoamine Oxidase A (MAOA) ELISA Kit | MAOA elisa kit

Human Monoamine Oxidase A (MAOA) ELISA Kit

Cat. Num. AAA2097983

• Gene Names: MAOA; BRNRS; MAO-A
• Reactivity: Human
• Synonyms: Monoamine Oxidase A (MAOA); Human Monoamine Oxidase A (MAOA) ELISA Kit; MAO-A; Amine oxidase [flavin-containing] A; MAOA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Monoamine Oxidase A (MAOA).; No significant cross-reactivity or interference between Monoamine Oxidase A (MAOA) and analogues was observed.
• Sequence Length: 527

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.119ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monoamine Oxidase A (MAOA) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monoamine Oxidase A (MAOA) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAOA elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monoamine Oxidase A (MAOA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monoamine Oxidase A (MAOA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monoamine Oxidase A (MAOA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Monoamine Oxidase A (MAOA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MAOA elisa kit

Enzyme & Kinase; Metabolic pathway; Endocrinology; Neuroscience; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 187351
• NCBI GeneID: 4128
• Molecular Weight: 44,848 Da
• NCBI Official Full Name: monoamine oxidase A
• NCBI Official Synonym Full Names: monoamine oxidase A
• NCBI Official Symbol: MAOA
• NCBI Official Synonym Symbols: BRNRS; MAO-A
• NCBI Protein Information: amine oxidase [flavin-containing] A
• UniProt Protein Name: Amine oxidase [flavin-containing] A
• Protein Family: Amine oxidase
• UniProt Gene Name: MAOA
• UniProt Synonym Gene Names: MAO-A

NCBI Description

This gene is one of two neighboring gene family members that encode mitochondrial enzymes which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. Mutation of this gene results in Brunner syndrome. This gene has also been associated with a variety of other psychiatric disorders, including antisocial behavior. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

Uniprot Description

MAOA: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine. Defects in MAOA are the cause of Brunner syndrome (BRUNS). Brunner syndrome is a form of X-linked non- dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Belongs to the flavin monoamine oxidase family.

Protein type: Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - histidine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - tyrosine; EC 1.4.3.4; Membrane protein, integral; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: Xp11.3

Cellular Component: integral component of membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: amine oxidase activity

Biological Process: biogenic amine metabolic process; dopamine catabolic process; neurotransmitter catabolic process; neurotransmitter metabolic process

Disease: Brunner Syndrome