Neprilysin active protein

Recombinant Neprilysin

Cat. Num. AAA201172

• Gene Names: MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
• Applications: SDS-Page
• Purity: > 95% by SDS - PAGE
• Synonyms: Neprilysin; Recombinant Neprilysin; MME; CALLA; CD10; CMT2T; NEP; SCA43; SFE; Atriopeptidase; Common acute lymphocyticleukemia antigen; Enkephalinase; Neutral endopeptidase; Skin fibroblast elastase; Neprilysin active protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS - PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 0.1mM PMSF, 10% glycerol.
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: < ADP > YDDGICK SSDCIKSAAR LIQNMDATTE PCTDFFKYAC GGWLKRNVIPETSSRYGNFDILRDELEVVL KDVLQEPKTE DIVAVQKAKA LYRSCINESA IDSRGGEPLLKLLPDIYGWPVATENWEQKY GASWTAEKAI AQLNSKYGKK VLINLFVGTD DKNSVNHVIHIDQPRLGLPSRDYYECTGIY KEACTAYVDF MISVARLIRQ EERLPIDENQ LALEMNKVMELEKEIANATAKPEDRNDPML LYNKMTLAQI QNNFSLEING KPFSWLNFTN EIMSTVNISITNEEDVVVYAPEYLTKLKPI LTKYSARDLQ NLMSWRFIMD LVSSLSRTYK ESRNAFRKALYGTTSETATWRRCANYVNGN MENAVGRLYV EAAFAGESKH VVEDLIAQIR EVFIQTLDDLTWMDAETKKRAEEKALAIKE RIGYPDDIVS NDNKLNNEYL ELNYKEDEYF ENIIQNLKFSQSKQLKKLREKVDKDEWISG AAVVNAFYSS GRNQIVFPAG ILQPPFFSAQ QSNSLNYGGIGMVIGHEITHGFDDNGRNFN KDGDLVDWWT QQSASNFKEQ SQCMVYQYGN FSWDLAGGQHLNGINTLGENIADNGGLGQA YRAYQNYIKK NGEEKLLPGL DLNHKQLFFL NFAQVWCGTYRPEYAVNSIKTDVHSPGNFR IIGTLQNSAE FSEAFHCRKN SYMNPEKKCR VW < HHHHHH >
• Sequence Length: 750

• Applicable Applications for Neprilysin active protein: SDS-PAGE; Enzyme Activity
• Tag: His-Tag
• Biological Activity: Specific activity is > 5,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to MCA- Pro-Leu-OH per minute at pH 8.8 at 25C.
• Domain: 52-750aa
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing conditionandvisualized by coomassie blue stain)

Related Product Information for Neprilysin active protein

Neprilysin, also known as MME, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. It degrades the amyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. It is expressed in a wide variety of tissues and is particularly abundant in kidney. It is also a common acute lymphocytic leukemia antigen that is an important cell surface marker in the identification of human acute lymphocytic leukemia (ALL). It is of use in hematological identification since it is expressed by early B, pro-B and pre-B lymphocytes, and by lymph node germinal centers. Recombinant human neprilysin, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Neprilysin active protein

Immunology

References

Auer-Grumbach M., et al. (2016) Am J Hum Genet. 99:607-623.;;Hama E., et al. (2005) Med Hypotheses. 65:498-500.

NCBI and Uniprot Product Information

• NCBI GI #: 116256329
• NCBI GeneID: 4311
• NCBI Accession #: NP_000893.2
• NCBI GenBank Nucleotide #: NP_000893.2
• UniProt Accession #: P08473
• Molecular Weight: 80.9kDa (708aa)
• NCBI Official Full Name: neprilysin isoform a
• NCBI Official Synonym Full Names: membrane metalloendopeptidase
• NCBI Official Symbol: MME
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA; CMT2T; SCA43
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• Protein Family: Neprilysin
• UniProt Gene Name: MME
• UniProt Synonym Gene Names: EPN; CALLA; NEP; Neutral endopeptidase; SFE
• UniProt Entry Name: NEP_HUMAN

NCBI Description

The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017]