Rabbit anti-Human CHK Polyclonal Antibody | anti-CHK antibody

CHK, CT (Choline Kinase alpha, CK, CHETK-alpha, Ethanolamine Kinase, EK, CHKA, CKI) (MaxLight 550)

Cat. Num. AAA6468827

• Gene Names: CHKA; CK; EK; CHK; CKI
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: CHK; Polyclonal Antibody; CT (Choline Kinase alpha; CK; CHETK-alpha; Ethanolamine Kinase; EK; CHKA; CKI) (MaxLight 550); anti-CHK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHK.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-CHK antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 427-457 in the C-terminal region of human CHK, UniProt Accession #NP_001268; P35790.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysate (35ug/lane) usingMBS6000382. CHK(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CHK antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 47078276
• NCBI GeneID: 1119
• NCBI Accession #: NP_001268
• NCBI GenBank Nucleotide #: NM_001277
• UniProt Accession #: P35790
• Molecular Weight: 50kDa
• NCBI Official Full Name: choline kinase alpha isoform a
• NCBI Official Synonym Full Names: choline kinase alpha
• NCBI Official Symbol: CHKA
• NCBI Official Synonym Symbols: CK; EK; CHK; CKI
• NCBI Protein Information: choline kinase alpha
• UniProt Protein Name: Choline kinase alpha
• UniProt Gene Name: CHKA
• UniProt Synonym Gene Names: CHK; CKI; CK; EK
• UniProt Entry Name: CHKA_HUMAN

NCBI Description

The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CHKA: Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. Belongs to the choline/ethanolamine kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerophospholipid; EC 2.7.1.82; Kinase, other; EC 2.7.1.32

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cytosol

Molecular Function: choline binding; signal transducer activity; protein homodimerization activity; cholinesterase activity; drug binding; ethanolamine kinase activity; choline kinase activity; ATP binding

Biological Process: choline metabolic process; CDP-choline pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylcholine biosynthetic process; lipid metabolic process; signal transduction; lipid transport; phosphorylation