Western Blot (WB)
(Western blot analysis of PCDH18 in HepG2 cell lysate with PCDH18 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Rabbit PCDH18 Polyclonal Antibody | anti-PCDH18 antibody
PCDH18 Antibody
Gene Names
PCDH18; PCDH68L
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PCDH18 Antibody is affinity chromatography purified via peptide column.
Synonyms
PCDH18; Polyclonal Antibody; PCDH18 Antibody; PCDH68L; KIAA1562; Protocadherin-18; protocadherin 18; anti-PCDH18 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
At least three isoforms of PCDH18 are known to exist. This antibody is predicted to not cross-react with PCDH12.
Purity/Purification
PCDH18 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1135
Applicable Applications for anti-PCDH18 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
PCDH18 antibody can be used for detection of PCDH18 by Western blot at 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
PCDH18 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH18.
Buffer
PCDH18 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PCDH18 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of PCDH18 in HepG2 cell lysate with PCDH18 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of PCDH18 in HepG2 cell lysate with PCDH18 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of PCDH18 in mouse brain tissue with PCDH18 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of PCDH18 in mouse brain tissue with PCDH18 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of PCDH18 in Mouse Brain cells with PCDH18 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of PCDH18 in Mouse Brain cells with PCDH18 antibody at 20 μg/mL.)
Related Product Information for anti-PCDH18 antibody
PCDH18 Antibody: Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 18 (PCDH18) was initially identified through screening of human and mouse ESTs using conserved cytoplasmic domain motifs. Tissue screening revealed PCDH18 broad expression in tissues such as brain, liver, heart, kidney, lung, and trachea. PCDH18 expression is also temporally and spatially regulated in the mouse embryonic brain and interacts with Disabled-1, an intracellular adapter protein involved in neuronal migration and cell positioning during mammalian brain development. PCDH18 was also found to be expressed in the developing zebrafish neural tube and central nervous system, lending support to the hypothesis that PCDH18 may play a role during brain development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
126,021 Da
NCBI Official Full Name
protocadherin-18 isoform 1
NCBI Official Synonym Full Names
protocadherin 18
NCBI Official Symbol
PCDH18
NCBI Official Synonym Symbols
PCDH68L
NCBI Protein Information
protocadherin-18; protocadherin 68-like protein
UniProt Protein Name
Protocadherin-18
Protein Family
UniProt Gene Name
PCDH18
UniProt Synonym Gene Names
KIAA1562
UniProt Entry Name
PCD18_HUMAN
NCBI Description
This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains, a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined, the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain. [provided by RefSeq, Jul 2008]
Uniprot Description
PCDH18: Potential calcium-dependent cell-adhesion protein. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q31
Cellular Component: plasma membrane; integral to membrane
Molecular Function: calcium ion binding
Biological Process: brain development; homophilic cell adhesion
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Product Notes
The PCDH18 pcdh18 (Catalog #AAA150724) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCDH18 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PCDH18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). PCDH18 antibody can be used for detection of PCDH18 by Western blot at 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the PCDH18 pcdh18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCDH18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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