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Western Blot (WB)
(Anti-CHERP rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:104 kDa Observed band size:130 kDa)
Rabbit anti-Human CHERP Polyclonal Antibody | anti-CHERP antibody
Anti-CHERP Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
CHERP; Polyclonal Antibody; Anti-CHERP Antibody; Rabbit Polyclonal; CHERP Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-DAN16 Antibody; Anti-SCAF6 Antibody; Anti-SRA1 Antibody; anti-CHERP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CHERP
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-CHERP antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
ICC/IF: 1:100-1:500
IHC-P: 1:50-1:200
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human CHERP fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human CHERP fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CHERP rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:104 kDa Observed band size:130 kDa)
Western Blot (WB)
(Anti-CHERP rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:104 kDa Observed band size:130 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human CHERP in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CHERP in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CHERP in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CHERP in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of CHERP in HEK293 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CHERP polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of CHERP in HEK293 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CHERP polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Related Product Information for anti-CHERP antibody
Ca(2+) homeostasis endoplasmic reticulum protein (CHERP) is a ubiquitously expressed protein that has been proposed as a regulator of both major families of endoplasmic reticulum Ca(2+) channels, inositol 1, 4, 5-trisphosphate receptors (IP(3)Rs) and ryanodine receptors (RyRs), with resulting effects on mitotic cycling. CHERP, containing a Pro-rich region and a phosphorylated Ser/Arg-rich RS-like domain, is a novel Ca(2+)-dependent ALG-2-interactive target in the nucleus. Dysregulation of calcium homeostasis endoplasmic reticulum protein (CHERP) has been implicated in several cancers, such as neuroblastoma and colorectal cancer.
References
Wang Q, et al. (2019) U2-related proteins cherp and sr140 contribute to colorectal tumorigenesis via alternative splicing regulation. Int J Cancer 145 (10): 2728-2739.Lin-Moshier Y, et al. (2013) Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (cherp) in cellular calcium signaling. J Biol Chem 288 (1): 355-367.Laplante JM, et al. (2000) Cloning of human ca2+ homoeostasis endoplasmic reticulum protein (cherp): Regulated expression of antisense cdna depletes cherp, inhibits intracellular ca2+ mobilization and decreases cell proliferation. Biochem J 348 Pt 1 (Pt 1): 189-199.
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Product Notes
The CHERP (Catalog #AAA8108334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CHERP Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHERP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC-P: 1:50-1:200 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the CHERP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHERP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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