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Western Blot (WB)
(Anti-COPB2 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:102 kDa Observed band size:102 kDa)
Rabbit anti-Human COPB2 Polyclonal Antibody | anti-COPB2 antibody
Anti-COPB2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
COPB2; Polyclonal Antibody; Anti-COPB2 Antibody; Rabbit Polyclonal; COPB2 Antibody; Rabbit PAb; Antigen Affinity Purified; coatomer protein complex subunit beta 2 (beta prime); Anti-beta'-COP Antibody; anti-COPB2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human COPB2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 100mM Tris, 100mM Glycine, 10mM Nacl, 0.03%Proclin300, pH8.0
Applicable Applications for anti-COPB2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:200-1:1000
ICC/IF: 1:100-1:500
IHC-P: 1:200-1:1000
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human COPB2 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human COPB2 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-COPB2 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:102 kDa Observed band size:102 kDa)
Western Blot (WB)
(Anti-COPB2 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:102 kDa Observed band size:102 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human COPB2 in human stomach with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human COPB2 in human stomach with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human COPB2 in human gallbladder with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human COPB2 in human gallbladder with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of COPB2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human COPB2 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of COPB2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human COPB2 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Related Product Information for anti-COPB2 antibody
Coat proteins (COPs), including the major types clathrin, COPI and COPII, play a considerable role in intracellular transport by initiating the formation of transport vesicles. The coatomer protein complex subunit beta 2 (COPB2), also known as beta prime-COP or beta-Cop, is one of the seven subunits that form coatomer complex I. COPB2 serves as a mediator in the process of protein synthesis which transports proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. The upregulation of COPB2 in breast cancer is associated with age and lymph node metastasis in a validated cohort and promotes tumor cell proliferation and invasion. COPB2 is an oncogene in many cancer types. The upregulation of COPB2 in breast cancer is associated with age and lymph node metastasis in a validated cohort and promotes tumor cell proliferation and invasion. COPB2 could be a potential target gene in prostate cancer. COPB2 upregulation in lung adenocarcinoma cell lines facilitates cell growth and tumorigenesis via upregulating YAP1 expression. COPB2 plays a key role in the proliferation and cell cycle progression of human RKO and HCT116 colon cancer cells. COPB2 might be a potential therapeutic target for the treatment of human colon cancer.
References
Zhou Y, et al. (2020) High expression of copb2 predicts adverse outcomes: A potential therapeutic target for glioma. CNS Neurosci Ther 26 (3): 309-318.Wang Y, et al. (2018) Copb2 suppresses cell proliferation and induces cell cycle arrest in human colon cancer by regulating cell cycle-related proteins. Exp Ther Med 15 (1): 777-784.Bhandari A, et al. (2019) Copb2 is up-regulated in breast cancer and plays a vital role in the metastasis via n-cadherin and vimentin. J Cell Mol Med 23 (8): 5235-5245.
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Product Notes
The COPB2 (Catalog #AAA8108343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-COPB2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COPB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC-P: 1:200-1:1000 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the COPB2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COPB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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