Rabbit anti-Human, Mouse CHD7 Polyclonal Antibody | anti-CHD7 antibody

CHD7 Antibody

Cat. Num. AAA150915

• Gene Names: CHD7; CRG; HH5; IS3; KAL5
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: CHD7 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CHD7; Polyclonal Antibody; CHD7 Antibody; CRG; HH5; IS3; KAL5; KIAA1416; Chromodomain-helicase-DNA-binding protein 7; ATP-dependent helicase CHD7; CHD-7; chromodomain helicase DNA binding protein 7; anti-CHD7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Multiple isoforms of CHD7 are known to exist.
• Purity/Purification: CHD7 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 2997

• Applicable Applications for anti-CHD7 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: CHD7 antibody can be used for detection of CHD7 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CHD7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CHD7.
• Buffer: CHD7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CHD7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CHD7 in SK-N-SH cell lysate with CHD7 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CHD7 in mouse brain tissue with CHD7 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CHD7 in mouse brain tissue with CHD7 antibody at 20 μg/mL.)

Related Product Information for anti-CHD7 antibody

CHD7 Antibody: CHD proteins belong to a superfamily of proteins of ATP-dependent chromatin remodeling enzymes that have a unique combination of functional domains, including two N-terminal chromodomains, a SNF2-like ATPase/helicase domain and a DNA-binding domain. These proteins are thought to play a role in early embryonic development by affecting chromatin structure and gene expression. Mutations in one member of this family, CHD7, result in CHARGE syndrome. It colocalizes with embryonic stem (ES) cell master regulators OCT4/POU5F1, SOX2, and NANOG and is thought to modulate ES-specific gene transcription. Together with SOX2, CHD7 has been suggested to also regulate several human disease genes.

NCBI and Uniprot Product Information

• NCBI GI #: 54112403
• NCBI GeneID: 55636
• NCBI Accession #: NP_060250.2
• NCBI GenBank Nucleotide #: NM_017780.3
• UniProt Accession #: Q9P2D1; Q05DI5; Q2TAN4; Q66K35; Q7Z6C0; Q7Z7Q2; Q9NXA0; Q9NXA3; D0VBA5; E9PNZ2
• Molecular Weight: 101,085 Da
• NCBI Official Full Name: chromodomain-helicase-DNA-binding protein 7
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 7
• NCBI Official Symbol: CHD7
• NCBI Official Synonym Symbols: CRG; HH5; IS3; KAL5
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 7; CHARGE association; ATP-dependent helicase CHD7; chromodomain helicase DNA binding protein 7 isoform CRA_e
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 7
• Protein Family: Chromodomain-helicase-DNA-binding protein
• UniProt Gene Name: CHD7
• UniProt Synonym Gene Names: KIAA1416; CHD-7
• UniProt Entry Name: CHD7_HUMAN

NCBI Description

This gene encodes a protein that contains several helicase family domains. Mutations in this gene have been found in some patients with the CHARGE syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

CHD-7: Probable transcription regulator. May interact with CTCF. Interacts with CHD8. Widely expressed in fetal and adult tissues. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; DNA-binding; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 8q12.2

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding; helicase activity; ATP binding

Biological Process: limb development; heart morphogenesis; central nervous system development; olfactory behavior; blood circulation; positive regulation of multicellular organism growth; palate development; female genitalia development; regulation of growth hormone secretion; olfactory nerve development; embryonic hindlimb morphogenesis; adult walking behavior; sensory perception of sound; regulation of neurogenesis; regulation of transcription, DNA-dependent; skeletal development; T cell differentiation; adult heart development; inner ear morphogenesis; transcription, DNA-dependent; in utero embryonic development; olfactory bulb development; semicircular canal morphogenesis; cranial nerve development; chromatin modification; genitalia development; retina development in camera-type eye; artery morphogenesis; cognition; nose development; rRNA processing

Disease: Charge Syndrome; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Hypogonadotropic Hypogonadism 5 With Or Without Anosmia