Chromatin Immunoprecipitation (ChIP)
(BMI-1 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse embryonic fibroblast (MEF) cells and 4 ug BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify BMI-1 binding sites.)
Mouse anti-Human, Mouse BMI-1 Monoclonal Antibody | anti-BMI-1 antibody
BMI-1 antibody (mAb)(Clone AF27)
ChIP-Seq: 4ug per ChIP
WB: 0.5-2ug/ml
For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.
Chromatin Immunoprecipitation (ChIP)
(BMI-1 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse embryonic fibroblast (MEF) cells and 4 ug BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify BMI-1 binding sites.)
Chromatin Immunoprecipitation (ChIP)
(BMI-1 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse embryonic fibroblast (MEF) cells and 4 ug BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify BMI-1 binding sites.)
Western Blot (WB)
(BMI-1 antibody (mAb) tested by Western blot. 20 ug of K562 cell nuclear extract was run on SDS-PAGE and probed with BMI-1 antibody at 0.5 ug/ml. For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.)
Western Blot (WB)
(BMI-1 antibody (mAb) tested by Western blot. 20 ug of K562 cell nuclear extract was run on SDS-PAGE and probed with BMI-1 antibody at 0.5 ug/ml. For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.)
Background: BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.
NCBI and Uniprot Product Information
Uniprot Description
BMI1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.
Protein type: EC 6.3.2.-; Oncoprotein; Ubiquitin ligase; Motility/polarity/chemotaxis; Ligase; Ubiquitin conjugating system
Cellular Component: heterochromatin; nuclear body; cytoplasm; PcG protein complex; nucleus; ubiquitin ligase complex
Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; metal ion binding; chromatin binding
Biological Process: transcription, DNA-dependent; in utero embryonic development; embryonic skeletal development; positive regulation of immature T cell proliferation in the thymus; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; humoral immune response; embryonic skeletal morphogenesis; rostrocaudal neural tube patterning; regulation of transcription, DNA-dependent; regulation of catalytic activity; histone ubiquitination; DNA methylation; positive regulation of B cell proliferation; somatic stem cell division; histone acetylation; brain development; skeletal development; cellular process
Research Articles on BMI-1
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Product Notes
The BMI-1 bmi1 (Catalog #AAA388662) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BMI-1 antibody (mAb)(Clone AF27) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BMI-1 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Applications Validated: ChIP-Seq: 4ug per ChIP WB: 0.5-2ug/ml For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. Researchers should empirically determine the suitability of the BMI-1 bmi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMI-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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