Rabbit anti-Human, Mouse CHCHD3 Polyclonal Antibody | anti-CHCHD3 antibody

CHCHD3 Antibody

Cat. Num. AAA9603482

• Gene Names: CHCHD3; Mic19; MINOS3; PPP1R22
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CHCHD3; Polyclonal Antibody; CHCHD3 Antibody; CHCH3_HUMAN; CHCHD 3; Chchd3; Coiled coil helix coiled coil helix domain containing 3; Coiled coil helix coiled coil helix domain containing protein 3 mitochondrial; Coiled-coil-helix-coiled-coil-helix domain-containing protein 3; FLJ20420; MINOS3; mitochondrial; Mitochondrial inner membrane organizing system 3; PPP1R22; Protein phosphatase 1 regulatory subunit 22; anti-CHCHD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CHCHD3 antibody detects endogenous levels of CHCHD3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 232

• Applicable Applications for anti-CHCHD3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human CHCHD3.
• Subcellular Location: Mitochondrion Inner Membrane. Cytoplasm. Nucleus.
• Tissue Specificity: Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Similarity: Pig (92%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (100%), Dog (92%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of A431 cells, using CHCHD3 antibody.)

Immunohistochemistry (IHC)

(MBS9603482 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-CHCHD3 antibody

Function: Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:25781180).
Subunit Structure: Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MINOS1/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13 (PubMed:21081504, PubMed:22114354, PubMed:22228767, PubMed:25997101). This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9 (PubMed:21081504, PubMed:22114354, PubMed:22228767, PubMed:25997101). The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex. Interacts with HSPA1A/HSPA1B and OPA1, preferentially with the soluble OPA1 form (By similarity). Interacts with IMMT/MIC60.
Similarity: Belongs to the MICOS complex subunit Mic19 family. Metazoan Mic19 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 953768340
• NCBI GeneID: 54927
• NCBI Accession #: NP_001304106.1
• NCBI GenBank Nucleotide #: NM_001317177.1
• UniProt Accession #: Q9NX63
• Molecular Weight: Observed: 26 kDa; Predicted: 27 kDa
• NCBI Official Full Name: MICOS complex subunit MIC19 isoform 1
• NCBI Official Synonym Full Names: coiled-coil-helix-coiled-coil-helix domain containing 3
• NCBI Official Symbol: CHCHD3
• NCBI Official Synonym Symbols: Mic19; MINOS3; PPP1R22
• NCBI Protein Information: MICOS complex subunit MIC19
• UniProt Protein Name: MICOS complex subunit MIC19
• UniProt Gene Name: CHCHD3
• UniProt Synonym Gene Names: MIC19; MINOS3

NCBI Description

The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:25781180).