Rabbit anti-Human, Mouse Rad51B Polyclonal Antibody | anti-Rad51B antibody

Rad51B (G226) Polyclonal Antibody

Cat. Num. AAA3002756

• Gene Names: RAD51B; REC2; R51H2; RAD51L1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Rad51B; Polyclonal Antibody; Rad51B (G226) Polyclonal Antibody; DNA repair protein RAD51 homolog 2; R51H2; RAD51 homolog B; RAD51-like protein 1; RAD51B; RAD51L1; REC2; anti-Rad51B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rad51B (G226) polyclonal antibody detects endogenous levels of Rad51B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 350

• Applicable Applications for anti-Rad51B antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human Rad51B.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Rad51B (G226) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Rad51B (G226) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Rad51B (G226) pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Rad51B (G226) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-Rad51B antibody

The DNA repair proteins Rad50, Rad51A-D, Rad52 and Rad54 A and B are key components of the double-strand break repair pathway (DSBR). The process of homologous recombination is a major DNA repair pathway that operates on DNA double-strand breaks to promote error-free repair. Human Rad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. In addition to Rad51 protein, five paralogs have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3. Rad51B is a widely expressed protein that is involved in the repair of double-stranded DNA breaks that can arise during DNA replication. It can form a dimer with Rad51C and this complex shows single-stranded DNAdependent ATPase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 10835029
• NCBI GeneID: 5890
• NCBI Accession #: NP_002868.1
• NCBI GenBank Nucleotide #: NP_002868.1
• UniProt Accession #: O15315
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: DNA repair protein RAD51 homolog 2 isoform 1
• NCBI Official Synonym Full Names: RAD51 paralog B
• NCBI Official Symbol: RAD51B
• NCBI Official Synonym Symbols: REC2; R51H2; RAD51L1
• NCBI Protein Information: DNA repair protein RAD51 homolog 2
• UniProt Protein Name: DNA repair protein RAD51 homolog 2
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD51B
• UniProt Synonym Gene Names: RAD51L1; REC2; R51H2; Rad51B
• UniProt Entry Name: RA51B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. Rearrangements between this locus and high mobility group AT-hook 2 (HMGA2, GeneID 8091) have been observed in uterine leiomyomata. [provided by RefSeq, Mar 2016]

Uniprot Description

RAD51L1: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. The RAD51B- RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single- stranded gaps in duplex DNA and specifically to nicks in duplex DNA. A chromosomal aberration involving RAD51B is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23- 24) with HMGA1. A chromosomal aberration involving RAD51B is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with HMGA2. Belongs to the RecA family. RAD51 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 14q23-q24.2

Cellular Component: nucleoplasm; replication fork; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; DNA binding; four-way junction DNA binding; double-stranded DNA binding; single-stranded DNA binding; ATP binding

Biological Process: meiotic recombination; blood coagulation; DNA repair; double-strand break repair via homologous recombination; DNA recombination

Disease: Leiomyoma, Uterine