Rabbit anti-Human CHADL Polyclonal Antibody | anti-CHADL antibody

CHADL, ID (CHADL, SLRR4B, Chondroadherin-like protein) (Azide free) (HRP)

Cat. Num. AAA6285506

• Gene Names: CHADL; SLRR4B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHADL; Polyclonal Antibody; ID (CHADL; SLRR4B; Chondroadherin-like protein) (Azide free) (HRP); Chondroadherin-like protein; anti-CHADL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CHADL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CHADL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-434 amino acids from the Central region of human CHADL.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CHADL Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the CHADL antibody detected the CHADL protein (arrow).)

Related Product Information for anti-CHADL antibody

Members of the small leucine-rich proteoglycan (SLRP) family are mostly extracellular proteins that function upstream of multiple signaling cascades. They affect intracellular phosphorylation and modulate distinct pathways, such as those driven by Toll-like receptors, TGF? and receptor tyrosine kinases. As a member of the SLRP family, CHADL (Chondroadherin-like protein) is a 762 amino acid protein containing 21 LRR (leucine-rich) repeats, which promote protein-ligand interactions. Chondroadherin, a closely related protein, promotes attachment of chrondocytes, osteoblasts and fibroblasts and also plays an important role in the regulation of chrodrocyte proliferation and growth. CHADL is a secreted protein that is located in the extracellular space. There are two isoforms of CHADL that exist as a result of alternative splicing events.

Product Categories/Family for anti-CHADL antibody

Antibodies; Toll Like Receptors (TLR)

References

Barbe, L., et al. Mol. Cell Proteomics 7(3):499-508(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 194440719
• NCBI GeneID: 150356
• NCBI Accession #: NP_612490.1
• NCBI GenBank Nucleotide #: NM_138481.1
• UniProt Accession #: Q6NUI6; Q05CY2; Q4G0S0; Q5JY13; Q86XY1; Q96E60
• Molecular Weight: 82,388 Da
• NCBI Official Full Name: chondroadherin-like protein
• NCBI Official Synonym Full Names: chondroadherin-like
• NCBI Official Symbol: CHADL
• NCBI Official Synonym Symbols: SLRR4B
• NCBI Protein Information: chondroadherin-like protein
• UniProt Protein Name: Chondroadherin-like protein
• Protein Family: Chondroadherin-like protein
• UniProt Gene Name: CHADL
• UniProt Synonym Gene Names: SLRR4B
• UniProt Entry Name: CHADL_HUMAN

Uniprot Description

CHADL: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class IV subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: collagen binding

Biological Process: negative regulation of chondrocyte differentiation