Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7051849 at dilution of 1:100)
Rabbit anti-Human CH25H Polyclonal Antibody | anti-CH25H antibody
CH25H Antibody
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7051849 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7051849 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7051849 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7051849 at dilution of 1:100)
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
Description
We uses the recombinant human CH25H protein (142-272AA) to immunize rabbits and then purify the rabbit antiserum through protein G to obtain the anti-CH25H antibody. This CH25H antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95%. It only reacts with human CH25H protein, an IFN-induced enzyme that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC) to reduce cholesterol accumulation. 25HC, a natural oxysterol, plays a role in regulating cholesterol homeostasis and sterol biosynthesis and exerts broad-spectrum antiviral function. And this anti-CH25H antibody can be used in ELISA and IHC for the detection of the CH25H protein.
NCBI and Uniprot Product Information
NCBI Description
This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]
Uniprot Description
CH25H: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25- hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Belongs to the sterol desaturase family.
Protein type: EC 1.14.99.38; Lipid Metabolism - primary bile acid biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase
Chromosomal Location of Human Ortholog: 10q23.31
Cellular Component: cytosol; endoplasmic reticulum membrane
Molecular Function: C-4 methylsterol oxidase activity; cholesterol 25-hydroxylase activity; steroid hydroxylase activity
Biological Process: bile acid biosynthetic process; cholesterol metabolic process; lipid metabolic process; sterol biosynthetic process
Research Articles on CH25H
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Product Notes
The CH25H ch25h (Catalog #AAA7051849) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CH25H Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CH25H can be used in a range of immunoassay formats including, but not limited to, ELSIA (EIA), Immunohistochemistry (IHC). IHC:1:20-1:200. Researchers should empirically determine the suitability of the CH25H ch25h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CH25H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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