Mouse Cholesterol-25-Hydroxylase (CH25H) ELISA Kit | CH25H elisa kit

Mouse Cholesterol-25-Hydroxylase (CH25H) ELISA Kit

Cat. Num. AAA2706088

• Gene Names: CH25H; C25H
• Reactivity: Mouse
• Synonyms: Cholesterol-25-Hydroxylase (CH25H); Mouse Cholesterol-25-Hydroxylase (CH25H) ELISA Kit; h25OH; Cholesterol 25-monooxygenase; CH25H elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cholesterol-25-Hydroxylase (CH25H).; No significant cross-reactivity or interference between Cholesterol-25-Hydroxylase (CH25H) and analogues was observed.
• Sequence Length: 272

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.27ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholesterol-25-Hydroxylase (CH25H) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholesterol-25-Hydroxylase (CH25H) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CH25H elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholesterol-25-Hydroxylase (CH25H). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholesterol-25-Hydroxylase (CH25H). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholesterol-25-Hydroxylase (CH25H), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholesterol-25-Hydroxylase (CH25H) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502499
• NCBI GeneID: 9023
• NCBI Accession #: NP_003947.1
• NCBI GenBank Nucleotide #: NM_003956.3
• UniProt Accession #: O95992; B2RBY3
• Molecular Weight: 31,745 Da
• NCBI Official Full Name: cholesterol 25-hydroxylase
• NCBI Official Synonym Full Names: cholesterol 25-hydroxylase
• NCBI Official Symbol: CH25H
• NCBI Official Synonym Symbols: C25H
• NCBI Protein Information: cholesterol 25-hydroxylase
• UniProt Protein Name: Cholesterol 25-hydroxylase
• Protein Family: Cholesterol 25-hydroxylase
• UniProt Gene Name: CH25H
• UniProt Synonym Gene Names: h25OH

NCBI Description

This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]

Uniprot Description

CH25H: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25- hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Belongs to the sterol desaturase family.

Protein type: EC 1.14.99.38; Lipid Metabolism - primary bile acid biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q23.31

Cellular Component: cytosol; endoplasmic reticulum membrane; integral component of membrane

Molecular Function: cholesterol 25-hydroxylase activity; iron ion binding; steroid hydroxylase activity

Biological Process: bile acid biosynthetic process; cholesterol metabolic process; lipid metabolic process; sterol biosynthetic process