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Western Blot (WB) (Anti-CGB/ HCG-Beta Antibody (C-term)at 1:2000 dilution + human placenta lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human CGB/HCG-Beta Polyclonal Antibody | anti-CGB antibody

CGB/HCG-Beta Antibody (C-term)

Gene Names
CGB; CGB3; CGB5; CGB7; CGB8; hCGB
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CGB/HCG-Beta; Polyclonal Antibody; CGB/HCG-Beta Antibody (C-term); Choriogonadotropin subunit beta; CG-beta; Chorionic gonadotrophin chain beta; CGB; CGB3; anti-CGB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CGB/HCG-Beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the C-terminal region of human CGB/HCG-Beta.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
137-165
Sequence Length
165
Applicable Applications for anti-CGB antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-CGB/ HCG-Beta Antibody (C-term)at 1:2000 dilution + human placenta lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-CGB/ HCG-Beta Antibody (C-term)at 1:2000 dilution + human placenta lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(CGB/HCG-Beta Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the CGB/HCG-Beta antibody detected the CGB/HCG-Beta protein (arrow).)

Western Blot (WB) (CGB/HCG-Beta Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the CGB/HCG-Beta antibody detected the CGB/HCG-Beta protein (arrow).)

Immunohistochemistry (IHC)

(CGB/HCG-Beta Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CGB/HCG-Beta Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (CGB/HCG-Beta Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CGB/HCG-Beta Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-CGB antibody
This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 3 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene.
Product Categories/Family for anti-CGB antibody
References
Cole, L.A. Placenta 31(8):653-664(2010) Ra, Y.J., et al. Interact Cardiovasc Thorac Surg 11(1):114-116(2010) Verma, B., et al. J. Immunol. 184(4):2156-2165(2010) Handschuh, K., et al. Placenta 30(12):1016-1022(2009) Reisenbichler, E.S., et al. Breast J 15(5):527-530(2009)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
Molecular Weight
17739
NCBI Official Full Name
choriogonadotropin subunit beta
NCBI Official Synonym Full Names
chorionic gonadotropin, beta polypeptide
NCBI Official Symbol
CGB
NCBI Official Synonym Symbols
CGB3; CGB5; CGB7; CGB8; hCGB
NCBI Protein Information
choriogonadotropin subunit beta
UniProt Protein Name
Choriogonadotropin subunit beta
Protein Family
UniProt Gene Name
CGB
UniProt Synonym Gene Names
CGB3; CG-beta
UniProt Entry Name
CGHB_HUMAN

NCBI Description

This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 3 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CGB: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Belongs to the glycoprotein hormones subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: cellular protein metabolic process; cell-cell signaling; apoptosis; peptide hormone processing; female gamete generation; signal transduction

Research Articles on CGB

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Product Notes

The CGB cgb (Catalog #AAA9214030) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 137-165. The CGB/HCG-Beta Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CGB/HCG-Beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the CGB cgb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CGB/HCG-Beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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