Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Large Intestine Tissue; Lane3: Rat Stomach Tissue; Lane4: Rat Liver Tissue.)
Rabbit anti-Human Cytochrome P450 Polyclonal Antibody | anti-CPR antibody
Polyclonal Antibody to Cytochrome P450 Reductase (CPR)
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Large Intestine Tissue; Lane3: Rat Stomach Tissue; Lane4: Rat Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Large Intestine Tissue; Lane3: Rat Stomach Tissue; Lane4: Rat Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CPR, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CPR, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
Protein type: EC 1.6.2.4; Endoplasmic reticulum; Mitochondrial; Oxidoreductase
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: endoplasmic reticulum membrane; integral component of membrane; intracellular membrane-bound organelle; membrane; mitochondrion
Molecular Function: cytochrome-b5 reductase activity, acting on NAD(P)H; electron transfer activity; enzyme binding; FAD binding; FMN binding; hydrolase activity; iron-cytochrome-c reductase activity; NADP binding; NADPH-hemoprotein reductase activity; nitric oxide dioxygenase activity; protein binding
Biological Process: carnitine metabolic process; cellular organofluorine metabolic process; cellular response to follicle-stimulating hormone stimulus; cellular response to peptide hormone stimulus; demethylation; fatty acid oxidation; flavonoid metabolic process; internal peptidyl-lysine acetylation; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of lipase activity; nitrate catabolic process; nitric oxide catabolic process; positive regulation of cholesterol biosynthetic process; positive regulation of chondrocyte differentiation; positive regulation of monooxygenase activity; positive regulation of smoothened signaling pathway; positive regulation of steroid hormone biosynthetic process; regulation of growth plate cartilage chondrocyte proliferation; response to drug; response to nutrient; xenobiotic metabolic process
Disease: Antley-bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis; Antley-bixler Syndrome Without Genital Anomalies Or Disordered Steroidogenesis; Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency
Research Articles on CPR
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Product Notes
The CPR por (Catalog #AAA2028728) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cytochrome P450 Reductase (CPR) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CPR por for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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