Immunofluorescence (IF)
(MBS9606367 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human, Mouse CG028 Polyclonal Antibody | anti-CG028 antibody
CG028 Antibody
Gene Names
CCZ1; CCZ1A; CGI-43; C7orf28A; H_DJ1163J12.2
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CG028; Polyclonal Antibody; CG028 Antibody; C7orf28A; C7orf28B; CGI 43; Chromosome 7 open reading frame 28A; Chromosome 7 open reading frame 28B; DKFZp586I1023; FLJ60592; H_DJ1163J12.2; H_NH0577018.2; Hypothetical protein LOC221960; Hypothetical protein LOC51622; MGC19819; UPF0550 protein C7orf28; anti-CG028 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CG028 antibody detects endogenous levels of total CG028
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
482
Applicable Applications for anti-CG028 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Lysosome
Predicted Cross Reactivity
Xenopus
Similarity
Xenopus (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9606367 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9606367 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells using CG028 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells using CG028 antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-CG028 antibody
Similarity: Belongs to the CCZ1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 56 kD
Predicted: 56 kDa
Predicted: 56 kDa
NCBI Official Full Name
vacuolar fusion protein CCZ1 homolog
NCBI Official Synonym Full Names
CCZ1 homolog, vacuolar protein trafficking and biogenesis associated
NCBI Official Symbol
CCZ1
NCBI Official Synonym Symbols
CCZ1A; CGI-43; C7orf28A; H_DJ1163J12.2
NCBI Protein Information
vacuolar fusion protein CCZ1 homolog
UniProt Protein Name
Vacuolar fusion protein CCZ1 homolog B
UniProt Gene Name
CCZ1B
UniProt Synonym Gene Names
C7orf28B
Research Articles on CG028
Similar Products
Product Notes
The CG028 ccz1b (Catalog #AAA9606367) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CG028 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CG028 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CG028 ccz1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CG028, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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