Rabbit CEP164 Polyclonal Antibody | anti-CEP164 antibody

CEP164 Antibody

Cat. Num. AAA9401767

• Gene Names: CEP164; NPHP15
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: CEP164; Polyclonal Antibody; CEP164 Antibody; Centrosomal protein 164; anti-CEP164 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 1460

• Applicable Applications for anti-CEP164 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against an 18 amino acid peptide near the amino terminus of human CEP164.
• Target Name: CEP164
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CEP164 in rat heart tissue lysate with CEP164 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CEP164 in mouse heart tissue with CEP164 antibody at 5 ug/mL.)

Related Product Information for anti-CEP164 antibody

CEP164 was initially identified as a centrosomal protein, but other studies have indicated that it also plays a role in the formation of primary cilia, the microtubule-based sensory antennae projecting from the surface of many eukaryotic cells as well as in DNA damage response acting as a mediator protein. CEP164 interacts with both ATR and ATM, proteins that trigger a number of cellular responses including the initiation of DNA damaged-induced cell cycle checkpoints. It is phosphorylated upon replication stress, ultraviolet (UV) radiation, and ionizing radiation; silencing of CEP164 significantly reduces the DNA damage-induced phosphorylation of several proteins in the DNA damage-activated signaling cascade and compromises cell survival after UV damage. At least two isoforms of CEP164 are known to exist.

Product Categories/Family for anti-CEP164 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 115648142
• NCBI GeneID: 22897
• NCBI Accession #: NP_055771
• NCBI GenBank Nucleotide #: NM_014956.4
• Molecular Weight: 163,544 Da
• NCBI Official Full Name: centrosomal protein of 164 kDa isoform 1
• NCBI Official Synonym Full Names: centrosomal protein 164kDa
• NCBI Official Symbol: CEP164
• NCBI Official Synonym Symbols: NPHP15
• NCBI Protein Information: centrosomal protein of 164 kDa
• UniProt Protein Name: Centrosomal protein of 164 kDa
• Protein Family: Centrosomal protein
• UniProt Gene Name: CEP164
• UniProt Synonym Gene Names: KIAA1052; NPHP15; Cep164
• UniProt Entry Name: CE164_HUMAN

NCBI Description

This gene encodes a centrosomal protein involved in microtubule organization, DNA damage response, and chromosome segregation. The encoded protein is required for assembly of primary cilia and localizes to mature centrioles. Defects in this gene are a cause of nephronophthisis-related ciliopathies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

CEP164: Plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1. Defects in CEP164 can be a cause of nephronophthisis-related ciliopathies (NPHP-RC), a group of recessive diseases that affect kidney, retina and brain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: centriole; nucleoplasm; centrosome; extracellular space; cytosol

Molecular Function: protein binding

Biological Process: mitosis; cell division; organelle organization and biogenesis; cilium biogenesis; mitotic cell cycle; DNA repair; G2/M transition of mitotic cell cycle

Disease: Nephronophthisis 15