Rabbit anti-Human CE164 Polyclonal Antibody | anti-CE164 antibody

CE164, NT (CEP164, KIAA1052, Centrosomal protein of 164kD) (PE)

Cat. Num. AAA6284907

• Gene Names: CEP164; NPHP15
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CE164; Polyclonal Antibody; NT (CEP164; KIAA1052; Centrosomal protein of 164kD) (PE); CEP164; Centrosomal protein of 164kD; anti-CE164 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 1460

• Applicable Applications for anti-CE164 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CE164 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-274 amino acids from the N-terminal region of human CE164.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CE164 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CE164 antibody detected the CE164 protein (arrow).)

Flow Cytometry (FC/FACS)

(CE164 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CE164 antibody

Plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA,CHK2 and CHK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHK1.

Product Categories/Family for anti-CE164 antibody

Antibodies; Centrosomal Proteins

References

Pan, Y.R., et al. Cell Cycle 8(4):655-664(2009). Sivasubramaniam, S., et al. Genes Dev. 22(5):587-600(2008). Graser, S., et al. J. Cell Biol. 179(2):321-330(2007). Petretti, C., et al. EMBO Rep. 7(4):418-424(2006). Zick, A., et al. Nucleic Acids Res. 33(13):4235-4242(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 115648142
• NCBI GeneID: 22897
• NCBI Accession #: NP_055771.4
• NCBI GenBank Nucleotide #: NP_055771.4
• UniProt Accession #: Q9UPV0
• NCBI Official Full Name: centrosomal protein of 164 kDa isoform 1
• NCBI Official Synonym Full Names: centrosomal protein 164
• NCBI Official Symbol: CEP164
• NCBI Official Synonym Symbols: NPHP15
• NCBI Protein Information: centrosomal protein of 164 kDa
• UniProt Protein Name: Centrosomal protein of 164 kDa
• UniProt Gene Name: CEP164
• UniProt Synonym Gene Names: KIAA1052; NPHP15; Cep164
• UniProt Entry Name: CE164_HUMAN

NCBI Description

This gene encodes a centrosomal protein involved in microtubule organization, DNA damage response, and chromosome segregation. The encoded protein is required for assembly of primary cilia and localizes to mature centrioles. Defects in this gene are a cause of nephronophthisis-related ciliopathies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

CEP164: Plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1. Defects in CEP164 can be a cause of nephronophthisis-related ciliopathies (NPHP-RC), a group of recessive diseases that affect kidney, retina and brain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: nucleoplasm; centriole; centrosome; extracellular space; cytosol

Molecular Function: protein binding

Biological Process: mitosis; cell division; organelle organization and biogenesis; cilium biogenesis; mitotic cell cycle; DNA repair; G2/M transition of mitotic cell cycle

Disease: Nephronophthisis 15