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Western Blot (WB)
(Western Blot: Sample: Lane1: Fetal Mouse Tissue; Lane2: Human Hela Cells; Lane3: Human 293T Cells.)
Rabbit anti-Mouse Centromere Protein H Polyclonal Antibody | anti-CENPH antibody
Polyclonal Antibody to Centromere Protein H (CENPH)
Gene Names
Cenph; ENP; CENP-H; AU044255; 1700021I11Rik; 2410018A12Rik; 2610042E16Rik; 2810046K12Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Centromere Protein H; Polyclonal Antibody; Polyclonal Antibody to Centromere Protein H (CENPH); anti-CENPH antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CENPH. It has beenselected for its ability to recognize CENPH in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- ERI EDRISLLLRL RAQTKQQLLE YKSMIDTNEE KTPEQIMQEK QIEVKIEELE NEIEDVKSNI EMKSLALSRM KLSVALRDNM ENMGPENCVL TDDMKHILKL QKLIMKSQEE SSELEKKLLD VRKKRLQLKQ ASRSKLLEIQ IEKNKQKEDV DKMENSEMIK TMKKKLQTEI KITTVVQHTF QGLILASKTN WAEDPALRET
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- ERI EDRISLLLRL RAQTKQQLLE YKSMIDTNEE KTPEQIMQEK QIEVKIEELE NEIEDVKSNI EMKSLALSRM KLSVALRDNM ENMGPENCVL TDDMKHILKL QKLIMKSQEE SSELEKKLLD VRKKRLQLKQ ASRSKLLEIQ IEKNKQKEDV DKMENSEMIK TMKKKLQTEI KITTVVQHTF QGLILASKTN WAEDPALRET
Sequence Length
247
Applicable Applications for anti-CENPH antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-5ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
CENPH (Glu28~Leu241)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant CENPH (Glu28~Leu241) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040266
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Fetal Mouse Tissue; Lane2: Human Hela Cells; Lane3: Human 293T Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Fetal Mouse Tissue; Lane2: Human Hela Cells; Lane3: Human 293T Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CENPH, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CENPH, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,135 Da
NCBI Official Full Name
centromere protein H
NCBI Official Synonym Full Names
centromere protein H
NCBI Official Symbol
Cenph
NCBI Official Synonym Symbols
ENP; CENP-H; AU044255; 1700021I11Rik; 2410018A12Rik; 2610042E16Rik; 2810046K12Rik
NCBI Protein Information
centromere protein H
UniProt Protein Name
Centromere protein H
Protein Family
UniProt Gene Name
Cenph
UniProt Synonym Gene Names
CENP-H
Uniprot Description
CENPH: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Belongs to the centromere protein H family.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 13 53.23 cM|13 D1
Cellular Component: chromosome; chromosome, centromeric region; kinetochore; nucleolus; nucleus
Molecular Function: kinetochore binding
Biological Process: chromosome segregation; kinetochore assembly; kinetochore organization and biogenesis; mitotic cell cycle
Research Articles on CENPH
Similar Products
Product Notes
The CENPH cenph (Catalog #AAA2028089) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Centromere Protein H (CENPH) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Centromere Protein H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-5ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CENPH cenph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- ERI EDRISLLLRL RAQTKQQLLE YKSMIDTNEE KTPEQIMQEK QIEVKIEELE NEIEDVKSNI EMKSLALSRM KLSVALRDNM ENMGPENCVL TDDMKHILKL QKLIMKSQEE SSELEKKLLD VRKKRLQLKQ ASRSKLLEIQ IEKNKQKEDV DKMENSEMIK TMKKKLQTEI KITTVVQHTF QGLILASKTN WAEDPALRET. It is sometimes possible for the material contained within the vial of "Centromere Protein H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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