Western Blot (WB)
(Western Blot analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.)
Mouse anti-Human CENP-A Monoclonal Antibody | anti-CENPA antibody
CENP-A Antibody: ATTO 390
Western Blot (WB)
(Western Blot analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.)
Western Blot (WB)
(Western Blot analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.)
Scientific Background: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
NCBI and Uniprot Product Information
NCBI Description
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 2p23.3
Cellular Component: nucleoplasm; inner kinetochore of condensed chromosome; nucleosome; cytosol; nucleus; chromosome, pericentric region; condensed nuclear chromosome, pericentric region
Molecular Function: protein binding; DNA binding; protein heterodimerization activity; chromatin binding
Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; viral reproduction; establishment of mitotic spindle orientation; mitotic cell cycle
Research Articles on CENPA
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Product Notes
The CENPA cenpa (Catalog #AAA804105) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CENP-An Antibody: ATTO 390 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CENP-A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). 1:1000 (WB). Researchers should empirically determine the suitability of the CENPA cenpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENP-A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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