Rabbit anti-Human CENP-A Polyclonal Antibody | anti-CENPA antibody

CENP-A (Centromere Potein A)

Cat. Num. AAA610667

• Gene Names: CENPA; CenH3; CENP-A
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by peptide affinity chromatography.
• Synonyms: CENP-A; Polyclonal Antibody; CENP-A (Centromere Potein A); Anti -CENP-A (Centromere Potein A); anti-CENPA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total human CENP-A protein. This antibody does not cross-react with other histone proteins, including Histone H3.
• Purity/Purification: Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-CENPA antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot and Immunofluorescence.; Dilution: Western Blot: 1:1000; Immunofluorescence:1:400
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to human CENP-A protein.
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of various whole cell lysates using MBS610667 CENP-A Antibody.)

Testing Data

Related Product Information for anti-CENPA antibody

Modulation of chromatin structure plays a critical role in the regulation of transcription and replication of the genome in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure (1). CENP-A, also known as the chromatin-associated protein CSE4 (capping-enzyme suppressor 4-p), is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin (2). The greatest divergence between CENP-A and canonical histone H3 occurs in the aminoterminal tail of the protein, which binds linker DNA between nucleosomes and facilitates proper folding of centromeric heterochromatin (3). The amino-terminal tail of CENP-A is also required for recruitment of other centromeric proteins (CENP-C, hSMC1, hZW10), proper kinetochore assembly and chromosome segregation during mitosis (4). Additional sequence divergence in the histone fold domain is responsible for correct targeting of CENP-A to the centromere (5). Many of the functions of CENP-A are regulated by phosphorylation (6,7). Aurora A-dependent phosphorylation of CENP-A on Ser7 during prophase is required for proper targeting of Aurora B to the inner centromere in prometaphase, proper kinetochore/microtubule attachment and proper alignment of chromosomes during mitosis (6). Specificity/Sensitivity: CENP-A Antibody detects endogenous levels of total human CENP-A protein. This antibody does not cross-react with other histone proteins, including Histone H3.

Product Categories/Family for anti-CENPA antibody

Antibodies; Abs to Centromeres

NCBI and Uniprot Product Information

• NCBI GI #: 1841749
• NCBI GeneID: 1058
• UniProt Accession #: P49450; Q53T74; Q9BVW2; D6W544
• Molecular Weight: 15,991 Da
• NCBI Official Full Name: centromere-specific histone H3 homolog
• NCBI Official Synonym Full Names: centromere protein A
• NCBI Official Symbol: CENPA
• NCBI Official Synonym Symbols: CenH3; CENP-A
• NCBI Protein Information: histone H3-like centromeric protein A; centromere autoantigen A; centromere protein A, 17kDa; centromere-specific histone
• UniProt Protein Name: Histone H3-like centromeric protein A
• Protein Family: CENP-A multicopy suppressor protein
• UniProt Gene Name: CENPA
• UniProt Synonym Gene Names: CENP-A
• UniProt Entry Name: CENPA_HUMAN

NCBI Description

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; inner kinetochore of condensed chromosome; nucleosome; cytosol; nucleus; condensed nuclear chromosome, pericentric region; chromosome, pericentric region

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; chromatin binding

Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; establishment of mitotic spindle orientation; viral reproduction; mitotic cell cycle