Rabbit anti-Human CENP-A Polyclonal Antibody | anti-CENPA antibody

CENP-A (Centromere Potein A)

Cat. Num. AAA613034

• Gene Names: CENPA; CenH3; CENP-A
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CENP-A; Polyclonal Antibody; CENP-A (Centromere Potein A); Anti -CENP-A (Centromere Potein A); anti-CENPA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CENP-A, Mr17kD. Broad species cross-reactivity predicted due to sequence conservation.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 5mg/ml BSA, 0.05% sodium azide before the addition of glycerol to 30%.

• Applicable Applications for anti-CENPA antibody: Western Blot (WB)
• Application Notes: Suitable for use in Immunoblot anaylsis.; Dilution: Immunoblot Analysis: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa acid extracted histones. 20ug control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2ug/ml anti-CENP-A. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunogen: Synthetic peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A..KLH conjugated
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot: 20ug Control Histones colcemid-treated (Lane 1) or untreated (Lane 2) were probed with MBS613034 (1:200).)

Related Product Information for anti-CENPA antibody

The kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle. The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner. Members of the first class include CENP-A, CENP-B, and CENP-C. The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex.

Product Categories/Family for anti-CENPA antibody

Antibodies; Abs to Centromeres

NCBI and Uniprot Product Information

• NCBI GI #: 1841749
• NCBI GeneID: 1058
• UniProt Accession #: P49450; Q53T74; Q9BVW2; D6W544
• Molecular Weight: 15,991 Da
• NCBI Official Full Name: centromere-specific histone H3 homolog
• NCBI Official Synonym Full Names: centromere protein A
• NCBI Official Symbol: CENPA
• NCBI Official Synonym Symbols: CenH3; CENP-A
• NCBI Protein Information: histone H3-like centromeric protein A; centromere autoantigen A; centromere protein A, 17kDa; centromere-specific histone
• UniProt Protein Name: Histone H3-like centromeric protein A
• Protein Family: CENP-A multicopy suppressor protein
• UniProt Gene Name: CENPA
• UniProt Synonym Gene Names: CENP-A
• UniProt Entry Name: CENPA_HUMAN

NCBI Description

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; inner kinetochore of condensed chromosome; nucleosome; cytosol; nucleus; condensed nuclear chromosome, pericentric region; chromosome, pericentric region

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; chromatin binding

Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; establishment of mitotic spindle orientation; viral reproduction; mitotic cell cycle