Human Cathepsin V (CTSV) ELISA Kit | CTSV elisa kit

Human Cathepsin V (CTSV) ELISA Kit

Cat. Num. AAA2022944

• Gene Names: CTSV; CTSU; CATL2; CTSL2
• Reactivity: Human
• Synonyms: Cathepsin V (CTSV); Human Cathepsin V (CTSV) ELISA Kit; CTS-L2; CTSL2; CATL2; CTSU; CTS-V; Cathepsin L2; CTSV elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cathepsin V (CTSV).; No significant cross-reactivity or interference between Cathepsin V (CTSV) and analogues was observed.
• Sequence Length: 334

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin V (CTSV) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin V (CTSV) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSV elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin V (CTSV). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin V (CTSV). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin V (CTSV), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin V (CTSV) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CTSV elisa kit

Enzyme & Kinase; Tumor immunity; Dermatology

NCBI and Uniprot Product Information

• NCBI GI #: 3107915
• NCBI GeneID: 1515
• UniProt Accession #: O60911; O60233; Q2TB86; Q5T1U0
• Molecular Weight: 37,329 Da
• NCBI Official Full Name: cathepsin V
• NCBI Official Synonym Full Names: cathepsin V
• NCBI Official Symbol: CTSV
• NCBI Official Synonym Symbols: CTSU; CATL2; CTSL2
• NCBI Protein Information: cathepsin L2
• UniProt Protein Name: Cathepsin L2
• UniProt Gene Name: CTSV
• UniProt Synonym Gene Names: CATL2; CTSL2; CTSU
• UniProt Entry Name: CATL2_HUMAN

NCBI Description

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2011]

Uniprot Description

CTSL2: Cysteine protease. May have an important role in corneal physiology. Belongs to the peptidase C1 family.

Protein type: Secreted, signal peptide; EC 3.4.22.15; Secreted; Protease

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; lysosomal lumen; neuron projection; microvillus; apical part of cell; extracellular region; nucleolus; perikaryon; secretory granule; external side of plasma membrane

Molecular Function: protein binding; histone binding; cysteine-type endopeptidase activity; protein complex binding; kininogen binding; aminopeptidase activity; cysteine-type carboxypeptidase activity; peptide binding; cysteine-type peptidase activity

Biological Process: extracellular matrix organization and biogenesis; hair follicle morphogenesis; response to glucocorticoid stimulus; Sertoli cell differentiation; antigen processing and presentation of exogenous peptide antigen via MHC class II; multicellular organismal aging; decidualization; cellular response to starvation; proteolysis; protein autoprocessing; extracellular matrix disassembly; response to glucose stimulus; autophagic cell death; nerve development; spermatogenesis