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Western Blot (WB) (Fig.1. Western blot analysis of 293T Hela lysate, antibody was diluted at 1:2000. HRP, Goat Anti-Rabbit IgG secondary antibody was diluted at 1:20000.)

Rabbit anti-Human CDK6 Polyclonal Antibody | anti-CDK6 antibody

CDK6 Polyclonal Antibody

Gene Names
CDK6; MCPH12; PLSTIRE
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Synonyms
CDK6; Polyclonal Antibody; CDK6 Polyclonal Antibody; Rabbit Anti-Cdk6 Polyclonal Antibody; Cyclin-dependent kinase 6; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE; anti-CDK6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody detects endogenous levels of Cdk6
Purity/Purification
Affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form/Format
Liquid; PBS, pH 7.4, Containing 0.02% Sodium Azide As Preservative and 50% Glycerol As Stabilizer.
Concentration
1 mg/ml (varies by lot)
Sequence Length
326
Applicable Applications for anti-CDK6 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:500-1:200
ELISA: 1:10000-1:20000
Immunogen
Synthesized peptide derived from part region of human Cdk6 protein at amino acid sequence of 280-325
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig.1. Western blot analysis of 293T Hela lysate, antibody was diluted at 1:2000. HRP, Goat Anti-Rabbit IgG secondary antibody was diluted at 1:20000.)

Western Blot (WB) (Fig.1. Western blot analysis of 293T Hela lysate, antibody was diluted at 1:2000. HRP, Goat Anti-Rabbit IgG secondary antibody was diluted at 1:20000.)

Immunohistochemistry (IHC)

(Fig.2. Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200.)

Immunohistochemistry (IHC) (Fig.2. Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200.)

Immunohistochemistry (IHC)

(Fig.3. Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200.)

Immunohistochemistry (IHC) (Fig.3. Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200.)

Immunohistochemistry (IHC)

(Fig.4. Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200.)

Immunohistochemistry (IHC) (Fig.4. Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200.)

Immunohistochemistry (IHC)

(Fig.5. Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200.)

Immunohistochemistry (IHC) (Fig.5. Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200.)
Related Product Information for anti-CDK6 antibody
Cdk6 protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of Cdk6 protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cyclin-dependent kinase 6
NCBI Official Synonym Full Names
cyclin dependent kinase 6
NCBI Official Symbol
CDK6
NCBI Official Synonym Symbols
MCPH12; PLSTIRE
NCBI Protein Information
cyclin-dependent kinase 6
UniProt Protein Name
Cyclin-dependent kinase 6
Protein Family
UniProt Gene Name
CDK6
UniProt Synonym Gene Names
CDKN6
UniProt Entry Name
CDK6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017]

Research Articles on CDK6

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Product Notes

The CDK6 cdk6 (Catalog #AAA9708676) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDK6 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDK6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:500-1:2000 IHC-P: 1:500-1:200 ELISA: 1:10000-1:20000. Researchers should empirically determine the suitability of the CDK6 cdk6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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