Rabbit anti-Human Cdk6 Polyclonal Antibody | anti-Cdk6 antibody

Cdk6 Polyclonal Antibody

Cat. Num. AAA2549574

• Gene Names: CDK6; MCPH12; PLSTIRE
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity Purification
• Synonyms: Cdk6; Polyclonal Antibody; Cdk6 Polyclonal Antibody; Cyclin-dependent kinase 6 (EC 2.7.11.22) (Cell division protein kinase 6) (Serine/threonine-protein kinase PLSTIRE); anti-Cdk6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 326

• Applicable Applications for anti-Cdk6 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB: 1:500-2000; IHC: 1:500-200; ELISA: 1:10000-20000
• Immunogen: Synthetic peptide from human protein
• Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of 293T, Hela cells using Cdk6 Polyclonal Antibody at dilution of 1:2000.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human tonsil tissue using Cdk6 Polyclonal Antibody at dilution of 1:200.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human colon cancer tissue using Cdk6 Polyclonal Antibody at dilution of 1:200.)

Related Product Information for anti-Cdk6 antibody

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 223718134
• NCBI GeneID: 1021
• NCBI Accession #: NP_001138778.1
• NCBI GenBank Nucleotide #: NM_001145306.1
• UniProt Accession #: Q00534; A4D1G0
• Molecular Weight: 34kDa
• NCBI Official Full Name: cyclin-dependent kinase 6
• NCBI Official Synonym Full Names: cyclin dependent kinase 6
• NCBI Official Symbol: CDK6
• NCBI Official Synonym Symbols: MCPH12; PLSTIRE
• NCBI Protein Information: cyclin-dependent kinase 6
• UniProt Protein Name: Cyclin-dependent kinase 6
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK6
• UniProt Synonym Gene Names: CDKN6

NCBI Description

The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017]

Uniprot Description

CDK6: a protein kinase of the CDK family. Important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. Phosphorylates and regulates the activity of the Rb tumor suppressor protein. Overexpressed and/or disrupted by translocation in leukemias, lymphomas and other cancers and amplified in gliomas and rodent cancers.

Protein type: CDK family; CDK/CDK4 subfamily; CDK4 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.22; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 7q21.2

Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytoplasm; cytosol; nucleoplasm; nucleus; ruffle

Molecular Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; FBXO family protein binding; protein binding

Biological Process: astrocyte development; cell cycle arrest; cell dedifferentiation; cell division; cellular response to phorbol 13-acetate 12-myristate; dentate gyrus development; G1/S transition of mitotic cell cycle; generation of neurons; gliogenesis; lateral ventricle development; negative regulation of cell cycle; negative regulation of cell differentiation; negative regulation of cell proliferation; negative regulation of cellular senescence; negative regulation of epithelial cell proliferation; negative regulation of monocyte differentiation; negative regulation of myeloid cell differentiation; negative regulation of osteoblast differentiation; positive regulation of cell-matrix adhesion; positive regulation of fibroblast proliferation; protein amino acid phosphorylation; regulation of cell motility; regulation of erythrocyte differentiation; regulation of G2/M transition of mitotic cell cycle; regulation of gene expression; response to virus; signal transduction; type B pancreatic cell development

Disease: Microcephaly 12, Primary, Autosomal Recessive