Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Cyclin Dependent Kinase 4 (CDK4) Polyclonal Antibody | anti-CDK4 antibody
Polyclonal Antibody to Cyclin Dependent Kinase 4 (CDK4)
MGHHHHHHSG SEF-YEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGAAGGGLP VSTVREVALL RRLEAFEHPN VVRLMDVCAT SRTDRDIKVT LVFEHIDQDL RTYLDKAPPP GLPVETIKDL MRQFLSGLDF LHANCIVHRD LKPENILVTS NGTVKLADFG LARIYSYQMA LTPVVVTLWY RAPEVLLQST YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR EVSLPRGAFA PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYL
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
Uniprot Description
CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991.
Protein type: CDK family; CDK/CDK4 subfamily; CDK4 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.22; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 10|10 D3
Cellular Component: bicellular tight junction; chromatin; cyclin-dependent protein kinase holoenzyme complex; cytoplasm; cytosol; membrane; nuclear membrane; nucleolus; nucleus; perinuclear region of cytoplasm; protein complex; transcription factor complex
Molecular Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; kinase activity; nucleotide binding; protein binding; protein complex binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity
Biological Process: cell cycle; cell division; cellular response to insulin stimulus; cellular response to interleukin-4; cellular response to ionomycin; cellular response to phorbol 13-acetate 12-myristate; G1/S transition of mitotic cell cycle; negative regulation of cell cycle arrest; phosphorylation; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of cell size; positive regulation of fibroblast proliferation; positive regulation of G2/M transition of mitotic cell cycle; positive regulation of translation; protein amino acid phosphorylation; regulation of cell cycle; regulation of cell proliferation; regulation of G2/M transition of mitotic cell cycle; regulation of gene expression; regulation of insulin receptor signaling pathway; regulation of lipid biosynthetic process; regulation of lipid catabolic process; regulation of multicellular organism growth; response to lead ion; response to organic substance; response to testosterone stimulus; signal transduction
Research Articles on CDK4
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Product Notes
The CDK4 cdk4 (Catalog #AAA2004214) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cyclin Dependent Kinase 4 (CDK4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin Dependent Kinase 4 (CDK4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CDK4 cdk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-YEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGAAGGGLP VSTVREVALL RRLEAFEHPN VVRLMDVCAT SRTDRDIKVT LVFEHIDQDL RTYLDKAPPP GLPVETIKDL MRQFLSGLDF LHANCIVHRD LKPENILVTS NGTVKLADFG LARIYSYQMA LTPVVVTLWY RAPEVLLQST YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR EVSLPRGAFA PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYL. It is sometimes possible for the material contained within the vial of "Cyclin Dependent Kinase 4 (CDK4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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