Mouse CDK4 (Cyclin Dependent Kinase 4) ELISA Kit | CDK4 elisa kit

Mouse CDK4 (Cyclin Dependent Kinase 4) ELISA Kit

Cat. Num. AAA7612404

• Gene Names: Cdk4; Crk3
• Reactivity: Mouse
• Synonyms: CDK4 (Cyclin Dependent Kinase 4); Mouse CDK4 (Cyclin Dependent Kinase 4) ELISA Kit; Cyclin-dependent kinase 4; CRK3; Cell division protein kinase 4; PSK-J3; Crk3; Cdk4; CDK4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CDK4. No significant cross-reactivity or interference between CDK4 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDK4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 231727
• NCBI GeneID: 12567
• UniProt Accession #: P30285
• Molecular Weight: 33,751 Da
• NCBI Official Full Name: Cyclin-dependent kinase 4
• NCBI Official Synonym Full Names: cyclin-dependent kinase 4
• NCBI Official Symbol: Cdk4
• NCBI Official Synonym Symbols: Crk3
• NCBI Protein Information: cyclin-dependent kinase 4; PSK-J3; p34<PSK-J3>/cdk4; serine/threonine kinase; cyclin dependent kinase 4; cell division protein kinase 4
• UniProt Protein Name: Cyclin-dependent kinase 4
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk4
• UniProt Synonym Gene Names: Crk3
• UniProt Entry Name: CDK4_MOUSE

Uniprot Description

CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991.

Protein type: Cell cycle regulation; EC 2.7.11.22; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; CDK family; CDK/CDK4 subfamily; CDK4 subfamily

Cellular Component: transcription factor complex; nuclear membrane; tight junction; membrane; perinuclear region of cytoplasm; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleolus; chromatin; nucleus; cytosol

Molecular Function: transferase activity; protein serine/threonine kinase activity; cyclin binding; protein binding; cyclin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; protein complex binding; kinase activity; ATP binding; protein kinase activity

Biological Process: response to drug; positive regulation of cell size; positive regulation of translation; regulation of cell cycle; positive regulation of apoptosis; response to testosterone stimulus; signal transduction; cell cycle; protein amino acid phosphorylation; regulation of cell proliferation; response to organic substance; positive regulation of fibroblast proliferation; regulation of gene expression; cell division; positive regulation of cell proliferation; response to lead ion; phosphorylation; G1/S transition of mitotic cell cycle