Immunohistochemistry (IHC)
(Biotinylated (1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Restin/CLIP1 Antibody | anti-CLIP1 antibody
Goat anti-Restin/CLIP1, Biotinylated Antibody
Gene Names
CLIP1; RSN; CLIP; CYLN1; CLIP170; CLIP-170
Reactivity
HumanExpected from sequence similarity: Mouse, Rat, Dog, Pig
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Restin/CLIP1; Goat anti-Restin/CLIP1; Biotinylated Antibody; Goat Anti-Restin/CLIP1 Antibody; CLIP1; CAP-GLY domain containing linker protein 1; CLIP; CLIP-170; CLIP170; CYLN1; MGC131604; RSN; Reed-Steinberg cell-expressed intermediate filament-associated protein; cytoplasmic linker 1; restin; restin (Reed-Steinberg cell-expressed intermediate fil; anti-CLIP1 antibody
Reactivity
Human
Expected from sequence similarity: Mouse, Rat, Dog, Pig
Expected from sequence similarity: Mouse, Rat, Dog, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
1427
Applicable Applications for anti-CLIP1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:8000.
WB: Approx 160kDa band observed in Human Breast Cancer lysates. Recommended concentration: 1-3ug/ml.
WB: Approx 160kDa band observed in Human Breast Cancer lysates. Recommended concentration: 1-3ug/ml.
Immunogen Sequence
Peptide with sequence C-PHSTHHGSRGEERP., from the C Terminus of the protein sequence according to NP_002947.1; NP_937883.1.
Peptide Sequence + C
C-PHSTHHGSRGEERP.
Epitope
C Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
(Biotinylated (1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Immunohistochemistry (IHC)
(Biotinylated (1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
161kDa
NCBI Official Full Name
CAP-Gly domain-containing linker protein 1 isoform a
NCBI Official Synonym Full Names
CAP-Gly domain containing linker protein 1
NCBI Official Symbol
CLIP1
NCBI Official Synonym Symbols
RSN; CLIP; CYLN1; CLIP170; CLIP-170
NCBI Protein Information
CAP-Gly domain-containing linker protein 1
UniProt Protein Name
CAP-Gly domain-containing linker protein 1
Protein Family
UniProt Gene Name
CLIP1
UniProt Synonym Gene Names
CYLN1; RSN; CLIP-170
NCBI Description
The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.
Research Articles on CLIP1
Similar Products
Product Notes
The CLIP1 clip1 (Catalog #AAA423737) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Restin/CLIP1, Biotinylated Antibody reacts with Human Expected from sequence similarity: Mouse, Rat, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Restin/CLIP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Pep-ELISA: antibody detection limit dilution 1:8000. WB: Approx 160kDa band observed in Human Breast Cancer lysates. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the CLIP1 clip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Restin/CLIP1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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