Rabbit anti-Human CDK10 Polyclonal Antibody | anti-CDK10 antibody

CDK10, NT (CDK10, Cyclin-dependent kinase 10, Cell division protein kinase 10, Serine/threonine-protein kinase PISSLRE) (APC)

Cat. Num. AAA6284260

• Gene Names: CDK10; PISSLRE
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CDK10; Polyclonal Antibody; NT (CDK10; Cyclin-dependent kinase 10; Cell division protein kinase 10; Serine/threonine-protein kinase PISSLRE) (APC); Serine/threonine-protein kinase PISSLRE; anti-CDK10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CDK10 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CDK10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~44 amino acids from the N-terminal region of human CDK10.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDK10 antibody (N-term) pre-incubated with (Lane1) and without (Lane 2) blocking peptide in A375 cell line lysate. CDK10 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-CDK10 antibody

CDK10 belongs to the CDK subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. This kinase has been shown to play a role in cellular proliferation. Its function is limited to cell cycle G2-M phase.

Product Categories/Family for anti-CDK10 antibody

Antibodies; Protein Kinases

References

Crawford, J., et al., Genomics 56(1):90-97 (1999). Brambilla, R., et al., Oncogene 9(10):3037-3041 (1994). Grana, X., et al., Oncogene 9(7):2097-2103 (1994). Morgan, D. O. Annu. Rev. Cell Dev. Biol. 13, 261 (1997). Sherr, C. Science 274:1672 (1996). Kamb A. TIG 11:136 (1995) Zhang, H. et al, Cell 82, 915 (1995) Parge, HE. et al., Science 262, 387 (1993) Hershko, A. et al., Ann. Rev. Biochem. 61, 761 (1992) Peters, JM. Curr. Biol. 10, 759 (1998). Skowyra, D. etal., Cell 91, 209 (1997). Ganoth D. et al., Nature Cell Biol. 3, 321-324 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 237858580
• NCBI GeneID: 8558
• NCBI Accession #: NP_001092003.2
• NCBI GenBank Nucleotide #: NM_001098533.2
• UniProt Accession #: Q15131; Q0VGZ7; Q15130; Q6PJC0; A8MXU6; B7Z420; D3DX82; D3DX83
• Molecular Weight: 41,038 Da
• NCBI Official Full Name: cyclin-dependent kinase 10 isoform c
• NCBI Official Synonym Full Names: cyclin-dependent kinase 10
• NCBI Official Symbol: CDK10
• NCBI Official Synonym Symbols: PISSLRE
• NCBI Protein Information: cyclin-dependent kinase 10; CDC2-related protein kinase; cell division protein kinase 10; cyclin-dependent kinase (CDC2-like) 10; cyclin-dependent kinase related protein; serine/threonine protein kinase PISSLRE; serine/threonine-protein kinase PISSLRE
• UniProt Protein Name: Cyclin-dependent kinase 10
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK10
• UniProt Entry Name: CDK10_HUMAN

NCBI Description

The protein encoded by this gene belongs to the CDK subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. This kinase has been shown to play a role in cellular proliferation and its function is limited to cell cycle G2-M phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

CDK10: a CMGC kinase of the CDK family. Plays a role in cellular proliferation. Its function is limited to cell cycle G2-M phase. At least three alternatively spliced isoforms have been reported, two of which contain multiple non-AUG translation initiation sites.

Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; CMGC group; CDK family; CDK10 subfamily; CDK/CDK10 subfamily

Chromosomal Location of Human Ortholog: 16q24

Molecular Function: protein binding; cyclin-dependent protein kinase activity; ATP binding

Biological Process: negative regulation of cell proliferation; traversing start control point of mitotic cell cycle; positive regulation of MAPKKK cascade; protein amino acid phosphorylation