Rabbit anti-Human CDH10 Polyclonal Antibody | anti-CDH10 antibody

CDH10, NT (CDH10, Cadherin-10, T2-cadherin) (APC)

Cat. Num. AAA6284073

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Peptide Immunoaffinity Chromatography.
• Synonyms: CDH10; Polyclonal Antibody; NT (CDH10; Cadherin-10; T2-cadherin) (APC); T2-cadherin; anti-CDH10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Peptide Immunoaffinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CDH10 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CDH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33~62 amino acids from the N-terminal region of human CDH10.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDH10 Antibody (N-term) in CEM cell line lysates (35ug/lane). CDH10 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH10 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of CEM cells using Cadherin 10 (CDH10) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CDH10 antibody

CDH10 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance.

Product Categories/Family for anti-CDH10 antibody

Antibodies; Cadherin

References

Kools, P., FEBS Lett. 452 (3), 328-334 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 16306530
• NCBI GeneID: 1008
• NCBI Accession #: NP_006718.2
• NCBI GenBank Nucleotide #: NM_006727.3
• UniProt Accession #: Q9Y6N8; Q9ULB3
• Molecular Weight: 88,451 Da
• NCBI Official Full Name: cadherin-10 preproprotein
• NCBI Official Synonym Full Names: cadherin 10, type 2 (T2-cadherin)
• NCBI Official Symbol: CDH10
• NCBI Protein Information: cadherin-10; T2-cadherin
• UniProt Protein Name: Cadherin-10
• Protein Family: Cadherin
• UniProt Gene Name: CDH10
• UniProt Entry Name: CAD10_HUMAN

NCBI Description

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance. [provided by RefSeq, Jan 2011]

Uniprot Description

CDH10: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 5p14.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: calcium ion binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; homophilic cell adhesion