Western Blot (WB)
(Anti-LDHB Antibody (Y240) at 1:2000 dilution + human heart lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human LDHB(Y240).ctrl Polyclonal Antibody | anti-LDHB antibody
Phospho-HUMAN-LDHB(Y240).ctrl
Gene Names
LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
Reactivity
Human
Applications
Western Blot
Synonyms
LDHB(Y240).ctrl; Polyclonal Antibody; Phospho-HUMAN-LDHB(Y240).ctrl; Purified Rabbit Polyclonal Antibody (Pab); L-lactate dehydrogenase B chain; LDH-B; LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46; LDHB; anti-LDHB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 210-250 amino acids from human.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
334
Applicable Applications for anti-LDHB antibody
Western Blot (WB)
Application Notes
WB ~~ 1:2000
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-LDHB Antibody (Y240) at 1:2000 dilution + human heart lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-LDHB Antibody (Y240) at 1:2000 dilution + human heart lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Product Categories/Family for anti-LDHB antibody
References
Takeno T.,et al.Biochem. J. 257:921-924(1989).
Sakai I.,et al.Biochem. J. 248:933-936(1987).
Bienvenut W.V.,et al.Submitted (MAY-2005) to UniProtKB.
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
Scanlan M.J.,et al.Int. J. Cancer 83:456-464(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36638
NCBI Official Full Name
L-lactate dehydrogenase B chain isoform LDHB
NCBI Official Synonym Full Names
lactate dehydrogenase B
NCBI Official Symbol
LDHB
NCBI Official Synonym Symbols
LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
NCBI Protein Information
L-lactate dehydrogenase B chain
UniProt Protein Name
L-lactate dehydrogenase B chain
Protein Family
UniProt Gene Name
LDHB
UniProt Synonym Gene Names
LDH-B; LDH-H
UniProt Entry Name
LDHB_HUMAN
NCBI Description
This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Recent studies have shown that two protein isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism, and that these C-terminally distinct isoforms have different subcellular localization. Alternatively spliced transcript variants have also been found for this gene. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Oct 2015]
Research Articles on LDHB
Similar Products
Product Notes
The LDHB ldhb (Catalog #AAA9216321) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-HUMAN-LDHB(Y240).ctrl reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LDHB(Y240).ctrl can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:2000. Researchers should empirically determine the suitability of the LDHB ldhb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LDHB(Y240).ctrl, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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