Rabbit anti-Human CDH10 Polyclonal Antibody | anti-CDH10 antibody

CDH10, NT (CDH10, Cadherin-10, T2-cadherin) (Azide free) (HRP)

Cat. Num. AAA6284076

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Peptide Immunoaffinity Chromatography.
• Synonyms: CDH10; Polyclonal Antibody; NT (CDH10; Cadherin-10; T2-cadherin) (Azide free) (HRP); T2-cadherin; anti-CDH10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Peptide Immunoaffinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CDH10 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CDH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33~62 amino acids from the N-terminal region of human CDH10.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDH10 Antibody (N-term) in CEM cell line lysates (35ug/lane). CDH10 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH10 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of CEM cells using Cadherin 10 (CDH10) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CDH10 antibody

CDH10 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance.

Product Categories/Family for anti-CDH10 antibody

Antibodies; Cadherin

References

Kools, P., FEBS Lett. 452 (3), 328-334 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 16306530
• NCBI GeneID: 1008
• NCBI Accession #: NP_006718.2
• NCBI GenBank Nucleotide #: NM_006727.3
• UniProt Accession #: Q9Y6N8; Q9ULB3
• Molecular Weight: 88,451 Da
• NCBI Official Full Name: cadherin-10 preproprotein
• NCBI Official Synonym Full Names: cadherin 10, type 2 (T2-cadherin)
• NCBI Official Symbol: CDH10
• NCBI Protein Information: cadherin-10; T2-cadherin
• UniProt Protein Name: Cadherin-10
• Protein Family: Cadherin
• UniProt Gene Name: CDH10
• UniProt Entry Name: CAD10_HUMAN

NCBI Description

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance. [provided by RefSeq, Jan 2011]

Uniprot Description

CDH10: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 5p14.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: calcium ion binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; homophilic cell adhesion