Rabbit anti-Human CDC45L Polyclonal Antibody | anti-CDC45L antibody

CDC45L, ID (CDC45, CDC45L, CDC45L2, Cell division control protein 45 homolog, PORC-PI-1) (MaxLight 650)

Cat. Num. AAA6283959

• Gene Names: CDC45; CDC45L; CDC45L2; PORC-PI-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CDC45L; Polyclonal Antibody; ID (CDC45; CDC45L2; Cell division control protein 45 homolog; PORC-PI-1) (MaxLight 650); CDC45; PORC-PI-1; anti-CDC45L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CDC45L.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-CDC45L antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 236-263 in the central region of human CDC45L, UniProt Accession #O75419.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS642963. CDC45L (arrow) was detected usingMBS642963.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded testis tissue using MBS642963 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642963 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS642963 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CDC45L antibody

Antibodies; Cell Division Cycle (CDC)

References

1. Chowdhury, A., et al. Mol. Cell. Biol. 30(6):1495-1507(2010). 2. Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(37):15628-15632(2009). 3. Ballabeni, A., et al. J. Biol. Chem. 284(5):3028-3036(2009). 4. Enjuanes, A., et al. Cancer Res. 68(24):10178-10186(2008). 5. Hoskins, J.M., et al. Clin. Cancer Res. 14(6):1788-1796(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 6685264
• NCBI GeneID: 8318
• UniProt Accession #: O75419; O60856; Q20WK8; Q6UW54; Q9UP68; B4DDB4; B4DDU3; E9PDH7
• Molecular Weight: 566
• NCBI Official Full Name: Cell division control protein 45 homolog
• NCBI Official Synonym Full Names: cell division cycle 45
• NCBI Official Symbol: CDC45
• NCBI Official Synonym Symbols: CDC45L; CDC45L2; PORC-PI-1
• NCBI Protein Information: cell division control protein 45 homolog; human CDC45; CDC45-related protein; cell division cycle 45-like 2; cell division cycle 45 homolog; CDC45 cell division cycle 45 homolog
• UniProt Protein Name: Cell division control protein 45 homolog
• UniProt Gene Name: CDC45
• UniProt Synonym Gene Names: CDC45L; CDC45L2
• UniProt Entry Name: CDC45_HUMAN

NCBI Description

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

CDC45L: Required for initiation of chromosomal DNA replication. Associated with ORC2. Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. Belongs to the CDC45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus

Molecular Function: protein binding; 3'-5' DNA helicase activity; DNA replication origin binding; chromatin binding; single-stranded DNA binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; regulation of chromatin silencing at telomere; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication checkpoint; DNA replication; DNA duplex unwinding; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle; pre-replicative complex assembly