Mouse CTH Monoclonal Antibody | anti-CTH antibody

CTH (Cystathionase (Cystathionine gamma-Lyase), MGC9471) (FITC)

Cat. Num. AAA6177111

• Applications: Western Blot
• Purity: Purified
• Synonyms: CTH; Monoclonal Antibody; CTH (Cystathionase (Cystathionine gamma-Lyase); MGC9471) (FITC); Cystathionase (Cystathionine gamma-Lyase); MGC9471; anti-CTH antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4E1-1B7
• Specificity: Recognizes CTH.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 405

• Applicable Applications for anti-CTH antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: CTH (AAH15807, 1aa-405aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged CTH is approximately 1ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of CTH expression in transfected 293T cell line by CTH monoclonal antibody (M01), clone 4E1-1B7.Lane 1: CTH transfected lysate (Predicted MW: 44.5 KDa).Lane 2: Non-transfected lysate.)

Western Blot (WB)

(CTH monoclonal antibody (M01), clone 4E1-1B7. Western Blot analysis of CTH expression in Hela S3 NE.)

Western Blot (WB)

(CTH monoclonal antibody (M01), clone 4E1-1B7. Western Blot analysis of CTH expression in human liver.)

Related Product Information for anti-CTH antibody

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Product Categories/Family for anti-CTH antibody

Antibodies; Enzymes

References

1. Statins upregulate cystathionine gamma-lyase transcription and H2S generation via activating Akt signaling in macrophage.Xu Y, Du HP, Li J, Xu R, Wang YL, You SJ, Liu H, Wang F, Cao YJ, Liu CF, Hu LF.Pharmacol Res. 2014 Jun 18. pii: S1043-6618(14)00098-X. 2.Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling.Ida T, Sawa T, Ihara H, Tsuchiya Y, Watanabe Y, Kumagai Y, Suematsu M, Motohashi H, Fujii S, Matsunaga T, Yamamoto M, Ono K, Devarie-Baez NO, Xian M, Fukuto JM, Akaike TProc Natl Acad Sci U S A. 2014 May 27;111(21):7606-11. doi: 10.1073/pnas.1321232111. Epub 2014 Apr 14. 3.Inhibition of hydrogen sulfide production by gene silencing attenuates inflammatory activity of LPS-activated RAW264.7 cells. Badiei A, Rivers-Auty J, Ang AD, Bhatia MAppl Microbiol Biotechnol. 2013 Jul 10. 4.Decreased Endogenous Production of Hydrogen Sulfide Accelerates Atherosclerosis.Mani S, Li H, Untereiner A, Wu L, Yang G, Austin RC, Dickhout JD, Lhotak S, Meng QH, Wang RCirculation. 2013 May 23. 5.Hydrogen sulfide protects against cellular senescence via S-sulfhydration of Keap1 and activation of Nrf2.Yang G, Zhao K, Ju Y, Mani S, Cao Q, Puukila S, Khaper N, Wu L, Wang RAntioxid Redox Signal. 2013 May 20;18(15):1906-19. doi: 10.1089/ars.2012.4645. Epub 2013 Feb 7. 6.Is cystathionine gamma-lyase protein expressed in the heart? Fu M, Zhang W, Yang G, Wang R.Biochem Biophys Res Commun. 2012 Oct 30. doi:pii: S0006-291X (12)02076-1. 10.1016/ j.bbrc.2012.10.084. 7.Hydrogen Sulfide Inhibits the Translational Expression of Hypoxia-inducible Factor-1alpha.Wu B, Teng H, Yang G, Wu L, Wang R.Br J Pharmacol. 2012 Jul 25. doi: 10.1111/j.1476-5381. 2012.02113.x. 8.Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe.Dufton N, Natividad J, Verdu EF, Wallace JL.Sci Rep. 2012;2:499. Epub 2012 Jul 9. 9.Hydrogen sulfide producing enzymes in pregnancy and preeclampsia. Holwerda KM, Bos EM, Rajakumar A, Ris-Stalpers C, van Pampus MG, Timmer A, Erwich JJ, Faas MM, van Goor H, Lely AT.Placenta. 2012 Mar 3. 10.Increased neointimal formation in cystathionine gamma-lyase deficient mice: Role of hydrogen sulfide in ?5?]1-integrin and matrix metalloproteinase-2 expression in smooth muscle cells. Yang G, Li H, Tang G, Wu L, Zhao K, Cao Q, Xu C, Wang R.J Mol Cell Cardiol. 2011 Dec 19. 11. MicroRNA-21 represses human cystathionine gamma-lyase expression by targeting at specificity protein-1 in smooth muscle cells.Yang G, Pei Y, Cao Q, Wang R.J Cell Physiol. 2011 Oct 27. doi: 10.1002/jcp.24006. 12.Protective Role of Hydrogen Sulfide against Noise-Induced Cochlear Damage: A Chronic Intracochlear Infusion Model. Li X, Mao XB, Hei RY, Zhang ZB, Wen LT, Zhang PZ, Qiu JH, Qiao L.PLoS One. 2011;6(10):e26728. Epub 2011 Oct 26. 13.Specificity protein-1 as a critical regulator of human cystathionine gamma-lyase in smooth muscle cells.Yang G, Pei Y, Teng H, Cao Q, Wang R.J Biol Chem. 2011 Jun 9. 14.Hydrogen Sulfide Modulates Contractile Function in Rat Jejunum. Kasparek MS, Linden DR, Farrugia G, Sarr MG.J Surg Res. 2011 Apr 22. 15.Hydrogen sulphide synthesis in the rat and mouse gastrointestinal tract. Martin GR, McKnight GW, Dicay MS, Coffin CS, Ferraz JG, Wallace JL.Dig Liver Dis. 2009 Jun 29. 16.Endogenous and Exogenous Hydrogen Sulfide Promotes Resolution of Colitis in Rats.Wallace JL, Vong L, McKnight W, Dicay M, Martin GR.Gastroenterology. 2009 Aug;137(2):569-78, 578.e1. Epub 2009 Apr 16. 17.Hydrogen sulfide from adipose tissue is a novel insulin resistance regulator.Feng X, Chen Y, Zhao J, Tang C, Jiang Z, Geng B.Biochem Biophys Res Commun. 2009 Feb 27;380(1):153-159. Epub 2009 Jan 21. 18.Hydrogen sulfide protects rat lung from ischemia-reperfusion injury. Fua Z, Liua X, Geng B, Fanga L, Tang C.Life Sci. 2008 Jun 6;82(23-24):1196-1202. Epub 2008 May 15. 19.Hydrogen sulfide enhances ulcer healing in rats. Wallace JL, Dicay M, McKnight W, Martin GR.FASEB J. 2007 Dec;21(14):4070-6. Epub 2007 Jul 18. 20.Hydrogen sulfide is a novel prosecretory neuromodulator in the Guinea-pig and human colon. Schicho R, Krueger D, Zeller F, Von Weyhern CW, Frieling T, Kimura H, Ishii I, De Giorgio R, Campi B, Schemann M.Gastroenterology. 2006 Nov;131(5):1542-52. Epub 2006 Aug 18.

NCBI and Uniprot Product Information

• NCBI GI #: 16041833
• NCBI GeneID: 1491
• NCBI Official Full Name: Cystathionase (cystathionine gamma-lyase)
• NCBI Official Synonym Full Names: cystathionine gamma-lyase
• NCBI Official Symbol: CTH
• NCBI Protein Information: cystathionine gamma-lyase
• Protein Family: Cystathionine gamma-lyase

NCBI Description

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]